Department of Food Science and Human Nutrition , University of Illinois at Urbana-Champaign , 1302 West Pennsylvania Avenue , Urbana , Illinois 61801 , United States.
J Agric Food Chem. 2018 Dec 5;66(48):12651-12656. doi: 10.1021/acs.jafc.8b06136. Epub 2018 Nov 20.
Glutathione, a tripeptide antioxidant, has recently been shown to be either utilized or synthesized by Gram-positive bacteria, such as lactic acid bacteria. Glutathione plays an important role in countering environmental stress, such as oxidative stress. In this study, cellular activity regarding glutathione in Lactobacillus fermentum CECT 5716 is characterized. We demonstrate that L. fermentum CECT 5716 has a better survival rate in the presence of glutathione under both oxidative and metal stress. As L. fermentum CECT 5716 does not possess the ability to synthesize glutathione under the conditions tested, it shows the ability to uptake both reduced and oxidized glutathione from the environment, regenerate reduced glutathione from oxidized glutathione, and perform secretion of glutathione to the environment.
谷胱甘肽是一种三肽抗氧化剂，最近的研究表明，革兰氏阳性菌（如乳酸菌）可以利用或合成谷胱甘肽。谷胱甘肽在对抗环境压力（如氧化应激）方面起着重要作用。本研究对发酵乳杆菌 CECT5716 中的谷胱甘肽进行了细胞活性分析。我们证明，在氧化和金属应激下，添加谷胱甘肽可提高发酵乳杆菌 CECT5716 的存活率。由于在测试条件下，发酵乳杆菌 CECT5716 不具备合成谷胱甘肽的能力，因此它表现出从环境中摄取还原型和氧化型谷胱甘肽、将氧化型谷胱甘肽还原为还原型谷胱甘肽以及将谷胱甘肽分泌到环境中的能力。
