Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Ciudad Universitaria, Avda. Puerta de Hierro, s/n., 28040, Madrid, Spain.
Appl Microbiol Biotechnol. 2015 May;99(10):4343-53. doi: 10.1007/s00253-015-6429-0. Epub 2015 Feb 10.
Lactobacillus fermentum CECT 5716, isolated from human milk, has immunomodulatory, anti-inflammatory, and anti-infectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. In this work, some phenotypic properties of L. fermentum CECT 5716 were evaluated, and the genetic basis for the obtained results was searched for in the strain genome. L. fermentum CECT 5716 does not contain plasmids and showed neither bacteriocin nor biogenic amine biosynthesis ability but was able to produce organic acids, glutathione, riboflavin, and folates and to moderately stimulate the maturation of mouse dendritic cells. No prophages could be induced, and the strain was sensitive to all antibiotics proposed by European Food Safety Authority (EFSA) standards, while no transmissible genes potentially involved in antibiotic resistance were detected in its genome. Globally, there was an agreement between the phenotype properties of L. fermentum CECT 5716 and the genetic information contained in its genome.
从人乳中分离出的发酵乳杆菌 CECT5716 具有免疫调节、抗炎和抗感染特性，这已通过多项体外和体内试验得到证实，这表明其作为益生菌菌株具有很强的潜力。在这项工作中，评估了发酵乳杆菌 CECT5716 的一些表型特性，并在菌株基因组中寻找获得结果的遗传基础。发酵乳杆菌 CECT5716 不含质粒，既没有细菌素也没有生物胺合成能力，但能够产生有机酸、谷胱甘肽、核黄素和叶酸，并能适度刺激小鼠树突状细胞的成熟。不能诱导噬菌体，该菌株对欧洲食品安全局 (EFSA) 标准提出的所有抗生素均敏感，而在其基因组中未检测到可能与抗生素耐药性相关的可传播基因。总体而言，发酵乳杆菌 CECT5716 的表型特性与其基因组中包含的遗传信息之间存在一致性。
