School of Applied Sciences, KIIT University, Bhubaneswar, 751024, Odisha, India.
School of Biotechnology, KIIT University, Bhubaneswar, 751024, Odisha, India.
Chem Biol Interact. 2019 Jan 5;297:141-154. doi: 10.1016/j.cbi.2018.11.004. Epub 2018 Nov 10.
The present study reports the regulation of cytotoxicity of Cu doped ZnO nanoparticles in macrophages (RAW 264.7) due to altered physiochemical properties changes like electrical properties by controlled doping of Cu in ZnO. Cu-doped ZnO nanoparticles were prepared by High Energy Ball Milling technique (HEBM) and formed single phase ZnCuO (x = 0.0, 0.01, 0.02, 0.03) were called as pure ZnO, Cu1%, 2%, 3% respectively. Hexagonal wurtzite structure with size range of 22-26 nm was verified. FE-SEM with EDX analysis indicated the Cu doping effect on the surface morphology of ZnO. Zeta potential of ZnCuO was found to be elevated with increase in doping percentage of Cu (-36.6 mV to +18.2 mV). Dielectric constant was found to be decreased with increasing doping percentage. Increase in doping percentage enhanced cytotoxicity of ZnCuO in macrophages with LC50 of 62 μg/ml, 51 μg/ml, 40 μg/ml, 32 μg/ml. Granularity change of macrophages suggested doping influenced cellular uptake as consequence of zeta potential and dielectric properties changes. 3% Cu doped ZnO shown a higher ROS signal and apoptosis than 2% and 1% Cu doping with exhibition of ROS scavenging nature leading to apoptosis of prepared Cu doped ZnO nanoparticles. Our findings revealed mechanism of cytotoxicity of ZnCuO as a consequence of alteration in electric properties eliciting ROS scavenging leading to higher apoptosis with increasing doping percentage of Cu in ZnO.
本研究报告了由于 Cu 在 ZnO 中的掺杂导致物理化学性质（如电特性）的改变，从而调节 Cu 掺杂 ZnO 纳米粒子在巨噬细胞（RAW 264.7）中的细胞毒性。采用高能球磨技术（HEBM）制备了 Cu 掺杂 ZnO 纳米粒子，并形成单相 ZnCuO（x=0.0、0.01、0.02、0.03），分别称为纯 ZnO、Cu1%、2%、3%。证实了具有 22-26nm 尺寸范围的六方纤锌矿结构。FE-SEM 和 EDX 分析表明 Cu 掺杂对 ZnO 表面形态的影响。ZnCuO 的 zeta 电位随着 Cu 掺杂百分比的增加而升高（从-36.6 mV 增加到+18.2 mV）。介电常数随着掺杂百分比的增加而降低。随着掺杂百分比的增加，ZnCuO 在巨噬细胞中的细胞毒性增强，LC50 值分别为 62μg/ml、51μg/ml、40μg/ml、32μg/ml。巨噬细胞的粒度变化表明掺杂影响了细胞摄取，这是由于 zeta 电位和介电性质的变化。3% Cu 掺杂的 ZnO 比 2%和 1% Cu 掺杂的 ZnO 表现出更高的 ROS 信号和凋亡，表现出 ROS 清除特性，导致制备的 Cu 掺杂 ZnO 纳米粒子凋亡。我们的研究结果揭示了 ZnCuO 细胞毒性的机制，这是由于电特性的改变引发了 ROS 清除，从而导致随着 ZnO 中 Cu 掺杂百分比的增加，凋亡率更高。
