Qi Baiwen, Yu Aixi, Zhu Shaobo, Chen Biao, Li Yan
Department of Microorthopaedics, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
J Huazhong Univ Sci Technolog Med Sci. 2010 Feb;30(1):89-93. doi: 10.1007/s11596-010-0116-2. Epub 2010 Feb 14.
In order to investigate the strength, structure and cell cytocompatibility of injectable thermosensitive chitosan (CS)/poly(vinyl alcohol) (PVA) composite hydrogel, chitosan hydrochloride solution was transferred to a neutral pH and mixed with different proportions of PVA, then the gelation time and strength of these different hydrogels were tested and spatial structures were observed under a scanning electron microscopy (SEM) after freeze-drying. The cytocompatibility of the hydrogels was evaluated through cytotoxicity test and three-dimensional culture with bone marrow mesenchymal stem cells. The results showed that the CS/PVA solution kept in liquid state at low temperature (0-4 degrees C) and turned into transparent elastomer about 15-20 min at 37 degrees C. Gelation time was prolonged, the strength increased and porous structure became dense with the PVA content increased in the mixed hydrogel. The cytotoxicity grades of these gels were from 0 to 1. Rabbit bone marrow mesenchymal stem cells could survive and proliferate in the gel within 3 weeks, and the gel had good cytocompatibility. It was concluded that thermosensitive CS/PVA composite hydrogel not only has interpenetrating network structure and better mechanical strength, but also has good cytocompatibility, and may be used as an injectable scaffold for tissue engineering.
为了研究可注射温敏壳聚糖（CS）/聚乙烯醇（PVA）复合水凝胶的强度、结构及细胞相容性，将盐酸壳聚糖溶液调节至中性pH值，并与不同比例的PVA混合，然后测试这些不同水凝胶的凝胶化时间和强度，并在冻干后通过扫描电子显微镜（SEM）观察其空间结构。通过细胞毒性试验和与骨髓间充质干细胞的三维培养来评估水凝胶的细胞相容性。结果表明，CS/PVA溶液在低温（0-4℃）下保持液态，在37℃时约15-20分钟转变为透明弹性体。随着混合水凝胶中PVA含量的增加，凝胶化时间延长，强度增加，多孔结构变得致密。这些凝胶的细胞毒性等级为0至1级。兔骨髓间充质干细胞可在凝胶中存活并增殖3周，且该凝胶具有良好的细胞相容性。结论是，温敏CS/PVA复合水凝胶不仅具有互穿网络结构和较好的机械强度，而且具有良好的细胞相容性，可作为组织工程的可注射支架材料。
