Department of Biomedical Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi 13488, Republic of Korea; Department of Biological Science, Korea University, Seoul 02841, Republic of Korea.
Center for Biomaterials, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:65-75. doi: 10.1016/j.msec.2018.09.011. Epub 2018 Sep 6.
Synthetic biodegradable polymers including poly(lactide-co-glycolide) (PLGA) have been widely used as alternatives to metallic implantable materials in the orthopedic field due to their superior biocompatibility and biodegradability. However, weak mechanical properties of the biodegradable polymers and inflammatory reaction caused by the acidic degradation products have limited their biomedical applications. In this study, we have developed a PLGA composite containing beta-tricalcium phosphate (β-TCP) and magnesium hydroxide (Mg(OH)) as additives to improve mechanical, osteoconductivity, and anti-inflammation property of the biopolymer composite simultaneously. The β-TCP has an osteoconductive effect and the Mg(OH) has a pH neutralizing effect. The PLGA/inorganic composites were uniformly blended via a twin extrusion process. The mechanical property of the PLGA/β-TCP/Mg(OH) composite was improved compared to the pure PLGA. In particular, the addition of Mg(OH) suppressed the inflammatory reaction of normal human osteoblast (NHOst) cells and also inhibited the differentiation of pre-osteoclastic cells into osteoclasts. Moreover, synergistically upregulated late osteogenic differentiation of NHOst cells was observed on the PLGA/β-TCP/Mg(OH) composite. Taken all together, we believe that the use of β-TCP and Mg(OH) as additives with synthetic biodegradable polymers has great potential by the synergistic effect in orthopedic applications.
合成可生物降解聚合物，包括聚（乳酸-共-乙醇酸）（PLGA），由于其优越的生物相容性和可生物降解性，已被广泛用作骨科领域中金属植入材料的替代品。然而，可生物降解聚合物的机械性能较弱，以及酸性降解产物引起的炎症反应，限制了它们在生物医学中的应用。在本研究中，我们开发了一种含有β-磷酸三钙（β-TCP）和氢氧化镁（Mg(OH)）作为添加剂的 PLGA 复合材料，以同时提高生物聚合物复合材料的机械性能、骨传导性和抗炎性能。β-TCP 具有骨传导作用，Mg(OH) 具有中和 pH 值的作用。PLGA/无机复合材料通过双螺杆挤出工艺均匀混合。与纯 PLGA 相比，PLGA/β-TCP/Mg(OH) 复合材料的机械性能得到了提高。特别是，Mg(OH) 的添加抑制了正常人类成骨细胞（NHOst）的炎症反应，并抑制了前破骨细胞向破骨细胞的分化。此外，还观察到 PLGA/β-TCP/Mg(OH) 复合材料上 NHOst 细胞的晚期成骨分化被协同上调。综上所述，我们相信使用β-TCP 和 Mg(OH) 作为合成可生物降解聚合物的添加剂具有很大的潜力，因为它们在骨科应用中有协同作用。
