The Key Laboratory of Luminescence and Real-time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
The Key Laboratory of Chongqing Inorganic Special Functional Materials, College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:858-866. doi: 10.1016/j.msec.2018.10.038. Epub 2018 Oct 9.
Here, we report the synthesis, characterization, and oxidase-like activity of CoO/CuO hollow nanocage (HNC) nanocomposites. The CoO/CuO HNCs were successfully prepared by hydrothermal treatment of ZIF-67 in copper nitrate solution, followed by carbonization in air. The obtained nanocomposites were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. The CoO/CuO HNCs possess enhanced oxidase-like activity compared with pure CoO and CuO, and they can catalytically oxidize the 3,3',5,5'-tetramethylbenzidine (TMB) substrate in the absence of HO to produce an intense blue product. Reactive oxygen species (ROS) measurements indicate that O and O radicals are the major ROS in catalytic oxidation of TMB by dissolved oxygen in the TMB-CoO/CuO HNC system. We then developed a simple, sensitive, visual, and colorimetric biosensing method for dopamine (DA) based on its inhibiting effect on TMB oxidation. The proposed method allows for DA detection with a limit of detection of 0.027 μM and a dynamic range of 0.05-8 μM. This new colorimetric method was successfully used to detect DA in biological samples. The present work demonstrates a simple strategy to fabricate an efficient oxidase mimic with potential applications in bioanalysis and clinical diagnosis.
在这里，我们报告了 CoO/CuO 空心纳米笼（HNC）纳米复合材料的合成、表征和氧化酶样活性。CoO/CuO HNC 是通过在硝酸铜溶液中对 ZIF-67 进行水热处理，然后在空气中碳化而成功制备的。所得纳米复合材料通过扫描电子显微镜、透射电子显微镜、X 射线光电子能谱和 X 射线衍射进行了表征。与纯 CoO 和 CuO 相比，CoO/CuO HNC 具有增强的氧化酶样活性，并且它们可以在没有 HO 的情况下催化氧化 3,3',5,5'-四甲基联苯胺（TMB）底物，生成强烈的蓝色产物。活性氧（ROS）测量表明，在 TMB-CoO/CuO HNC 体系中，溶解氧催化氧化 TMB 时，主要的 ROS 是 O 和 O 自由基。然后，我们基于多巴胺（DA）对 TMB 氧化的抑制作用，开发了一种简单、灵敏、可视化和比色生物传感方法来检测 DA。该方法允许检测到 0.027 μM 的 DA 检测限和 0.05-8 μM 的动态范围。这种新的比色方法成功地用于检测生物样品中的 DA。本工作展示了一种制造具有生物分析和临床诊断应用潜力的高效氧化酶模拟物的简单策略。
