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共掺杂 CeO/离子液体功能化的活性炭纳米复合材料用于过氧化物酶模拟物检测 HO 的比色生物传感

Co-Doped CeO/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of HO via Peroxidase Mimicking.

机构信息

Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, Pakistan.

Department of Chemistry, COMSATS University Islamabad, Park Road, Islamabad 45550, Pakistan.

出版信息

Molecules. 2023 Apr 9;28(8):3325. doi: 10.3390/molecules28083325.

DOI:10.3390/molecules28083325
PMID:37110559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10145388/
Abstract

Hydrogen peroxide acts as a byproduct of oxidative metabolism, and oxidative stress caused by its excess amount, causes different types of cancer. Thus, fast and cost-friendly analytical methods need to be developed for HO. Ionic liquid (IL)-coated cobalt (Co)-doped cerium oxide (CeO)/activated carbon (C) nanocomposite has been used to assess the peroxidase-like activity for the colorimetric detection of HO. Both activated C and IL have a synergistic effect on the electrical conductivity of the nanocomposites to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). The Co-doped CeO/activated C nanocomposite has been synthesized by the co-precipitation method and characterized by UV-Vis spectrophotometry, FTIR, SEM, EDX, Raman spectroscopy, and XRD. The prepared nanocomposite was functionalized with IL to avoid agglomeration. HO concentration, incubation time, pH, TMB concentration, and quantity of the capped nanocomposite were tuned. The proposed sensing probe gave a limit of detection of 1.3 × 10 M, a limit of quantification of 1.4 × 10 M, and an R of 0.999. The sensor gave a colorimetric response within 2 min at pH 6 at room temperature. The co-existing species did not show any interference during the sensing probe. The proposed sensor showed high sensitivity and selectivity and was used to detect HO in cancer patients' urine samples.

摘要

过氧化氢作为氧化代谢的副产物，其过量会导致氧化应激，从而引发不同类型的癌症。因此，需要开发快速且经济实惠的分析方法来检测 HO。本研究采用离子液体（IL）包覆的钴（Co）掺杂氧化铈（CeO）/活性炭（C）纳米复合材料来评估过氧化物酶样活性，用于 HO 的比色检测。活性炭和 IL 都对纳米复合材料的电导率具有协同作用，从而可以催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化。采用共沉淀法合成了 Co 掺杂的 CeO/活性炭纳米复合材料，并通过紫外-可见分光光度法、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、能谱（EDX）、拉曼光谱和 X 射线衍射（XRD）进行了表征。制备的纳米复合材料用 IL 功能化以避免团聚。调整了 HO 浓度、孵育时间、pH 值、TMB 浓度和封端纳米复合材料的用量。所提出的传感探针的检测限为 1.3×10 M，定量限为 1.4×10 M，R 为 0.999。该传感器在室温 pH 6 下 2 分钟内即可产生比色响应。在传感探针中，共存物质没有显示出任何干扰。该传感器具有高灵敏度和选择性，可用于检测癌症患者尿液样本中的 HO。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/91f66974952d/molecules-28-03325-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/7e73e9daf07e/molecules-28-03325-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/cdbf55c2d506/molecules-28-03325-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/afcac403f0ce/molecules-28-03325-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/383d472663de/molecules-28-03325-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/bf037388654f/molecules-28-03325-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/7e73e9daf07e/molecules-28-03325-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/cdbf55c2d506/molecules-28-03325-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0013/10145388/91f66974952d/molecules-28-03325-g013.jpg

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