Moussa Hassan I, Logan Megan, Wong Kingsley, Rao Zheng, Aucoin Marc G, Tsui Ting Y
Department of Chemical Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Waterloo Institute of Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Micromachines (Basel). 2018 Sep 13;9(9):464. doi: 10.3390/mi9090464.
Tantalum is one of the most important biomaterials used for surgical implant devices. However, little knowledge exists about how nanoscale-textured tantalum surfaces affect cell morphology. Mammalian (Vero) cell morphology on tantalum-coated comb structures was studied using high-resolution scanning electron microscopy and fluorescence microscopy. These structures contained parallel lines and trenches with equal widths in the range of 0.18 to 100 μm. Results showed that as much as 77% of adherent cell nuclei oriented within 10° of the line axes when deposited on comb structures with widths smaller than 10 μm. However, less than 20% of cells exhibited the same alignment performance on blanket tantalum films or structures with line widths larger than 50 μm. Two types of line-width-dependent cell morphology were observed. When line widths were smaller than 0.5 μm, nanometer-scale pseudopodia bridged across trench gaps without contacting the bottom surfaces. In contrast, pseudopodia structures covered the entire trench sidewalls and the trench bottom surfaces of comb structures with line-widths larger than 0.5 μm. Furthermore, results showed that when a single cell simultaneously adhered to multiple surface structures, the portion of the cell contacting each surface reflected the type of morphology observed for cells individually contacting the surfaces.
钽是用于外科植入装置的最重要生物材料之一。然而，关于纳米纹理化钽表面如何影响细胞形态的了解却很少。使用高分辨率扫描电子显微镜和荧光显微镜研究了钽涂层梳状结构上的哺乳动物（Vero）细胞形态。这些结构包含宽度在0.18至100μm范围内相等的平行线和沟槽。结果表明，当沉积在宽度小于10μm的梳状结构上时，多达77%的贴壁细胞核在线轴的10°范围内定向。然而，在钽薄膜或线宽大于50μm的结构上，只有不到20%的细胞表现出相同的排列性能。观察到两种类型的线宽依赖性细胞形态。当线宽小于0.5μm时，纳米级伪足跨越沟槽间隙而不接触底面。相反，伪足结构覆盖了线宽大于0.5μm的梳状结构的整个沟槽侧壁和沟槽底面。此外，结果表明，当单个细胞同时粘附到多个表面结构时，细胞与每个表面接触的部分反映了单独接触这些表面的细胞所观察到的形态类型。
