Eskandari Ali, Glerum D Moira, Tsui Ting Y
Department of Chemical Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Materials (Basel). 2022 May 17;15(10):3577. doi: 10.3390/ma15103577.
Cell adhesion is an essential biological function for division, migration, signaling and tissue development. While it has been demonstrated that this cell function can be modified by using nanometer-scale surface topographic structures, it remains unknown how contaminants such as indium (III) ion might influence this specific cell behavior. Herein, the influence of indium chloride on human dermal fibroblast (GM5565) adhesion characteristics was investigated, given the frequent contact of contaminants with skin. The morphology of the adherent cells and their mitochondrial reticulum was characterized on cell culture dishes and nanopatterned surfaces by using fluorescence confocal microscopy and scanning electron microscopy. Results showed a significant proportion of cells lost their ability to align preferentially along the line axes of the nanopattern upon exposure to 3.2 mM indium chloride, with cells aligned within 10° of the pattern line axes reduced by as much as ~70%. Concurrent with the cell adhesion behaviors, the mitochondria in cells exposed to indium chloride exhibit a punctate staining that contrasts with the normal network of elongated tubular geometry seen in control cells. Our results demonstrate that exposure to indium chloride has detrimental effects on the behavior of human fibroblasts and adversely impacts their mitochondrial morphology. This shows the importance of evaluating the biological impacts of indium compounds.
细胞黏附是细胞分裂、迁移、信号传导和组织发育所必需的生物学功能。虽然已经证明可以通过使用纳米级表面形貌结构来改变这种细胞功能，但诸如铟（III）离子等污染物如何影响这种特定的细胞行为仍不清楚。鉴于污染物经常与皮肤接触，本文研究了氯化铟对人皮肤成纤维细胞（GM5565）黏附特性的影响。通过荧光共聚焦显微镜和扫描电子显微镜对细胞培养皿和纳米图案化表面上贴壁细胞的形态及其线粒体网络进行了表征。结果显示，暴露于3.2 mM氯化铟后，相当一部分细胞失去了沿纳米图案线轴优先排列的能力，与图案线轴夹角在10°以内排列的细胞减少了约70%。与细胞黏附行为同时发生的是，暴露于氯化铟的细胞中的线粒体呈现点状染色，这与对照细胞中正常的细长管状几何网络形成对比。我们的结果表明，暴露于氯化铟对人成纤维细胞的行为有不利影响，并对其线粒体形态产生负面影响。这表明评估铟化合物的生物学影响的重要性。
