Domańska-Senderowska D, Snochowska A, Szmigielska P, Jastrzębski Z, Jegier A, Kiszałkiewicz J, Dróbka K, Jastrzębska J, Pastuszak-Lewandoska D, Cięszczyk P, Maciejewska-Skrendo A, Zmijewski P, Brzeziańska-Lasota E
Department of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, Lodz, Poland.
Faculty of Tourism and Recreation, Gdansk University of Physical Education and Sport, Gdansk, Poland.
Balkan J Med Genet. 2018 Oct 29;21(1):19-25. doi: 10.2478/bjmg-2018-0008. eCollection 2018 Jun.
The gene codes protein that belongs to the peroxisome proliferator-activated receptor (PPAR) family engaged in a variety of biological processes, including lipid metabolism in muscle cells. In this study, we assess the relationship between gene expression lipid metabolism parameters and the variation of the gene expression before (T) and after 12 hours of training (T) sessions in a group of football players. Peripheral blood lymphocytes were obtained from 22 football players (17.5±0.7 years, 178±0.7 cm, 68.05±9.18 kg). The gene expression, analyzed by quantitative polymerase chain reaction (qPCR), was significantly higher after T ( = 0.0006). Moreover, at the end of the training cycle, there was a significant decrease in relative fat tissue (FAT) (%) ( = 0.01) and absolute FAT (kg) ( = 0.01). A negative correlation was observed between absolute FAT (kg) and gene expression level in T ( = 0.03). The levels of cholesterol and triglyceride (TG) fractions were not significantly different ( >0.05) before and after training. No significant relationship between expression and cholesterol or TG levels was found. We found that physical training affects expression. Moreover, the negative correlation between expression and absolute FAT (kg) level may be indicative of the contribution of in metabolic adaptation to increased lipid uptake that can be used to control the body composition of athletes.
该基因编码的蛋白质属于过氧化物酶体增殖物激活受体（PPAR）家族，参与多种生物学过程，包括肌肉细胞中的脂质代谢。在本研究中，我们评估了一组足球运动员在训练前（T0）和训练12小时后（T12）基因表达、脂质代谢参数之间的关系以及该基因表达的变化。从22名足球运动员（年龄17.5±0.7岁，身高178±0.7厘米，体重68.05±9.18千克）获取外周血淋巴细胞。通过定量聚合酶链反应（qPCR）分析，该基因表达在T12后显著升高（P = 0.0006）。此外，在训练周期结束时，相对脂肪组织（FAT）（%）（P = 0.01）和绝对脂肪量（kg）（P = 0.01）显著降低。观察到绝对脂肪量（kg）与T12时该基因表达水平呈负相关（P = 0.03）。训练前后胆固醇和甘油三酯（TG）组分水平无显著差异（P>0.05）。未发现该基因表达与胆固醇或TG水平之间存在显著关系。我们发现体育训练会影响该基因表达。此外，该基因表达与绝对脂肪量（kg）水平之间的负相关可能表明该基因在代谢适应增加的脂质摄取中发挥作用，这可用于控制运动员的身体成分。
