Fernandes Andreína, Pesci-Feltri Adriana, García-Fleury Isabel, López Marco, Guida Vincent, De Macedo Marisol, Correnti María
Oncology and Hematology Institute, Caracas 1050, Venezuela.
University Hospital of Caracas, Caracas 1050, Venezuela.
World J Clin Oncol. 2018 Nov 10;9(7):123-132. doi: 10.5306/wjco.v9.i7.123.
To detect human papilloma virus (HPV) presence and to characterize cellular immune response in breast cancer patients.
A total of 74 women were included, of which 48 samples were from patients diagnosed with breast cancer and 26 patients with benign pathology of the breast. Molecular subtype classification was performed based on the immunohistochemical reports of the tumor piece. HPV genome detection and genotyping from fresh breast biopsies was performed using the INNO-LIPA HPV Genotyping Extra test (Innogenetics, Ghent, Belgium). CD3+, CD4+, CD8+ and natural killer (NK)+ cells levels from peripheral blood samples from patients with breast cancer and benign pathology were measured by flow cytometry.
Luminal A was the most frequent breast cancer molecular subtype (33.33%). HPV was detected in 25% of the breast cancer patients, and genotype 18 was the most frequent in the studied population. The mean of CD3+, CD4+ and CD8+ subpopulations were decreased in patients with breast cancer, in relation to those with benign pathology, with a statistically significant difference in CD8+ values ( = 0.048). The mean of NK+ cells was increased in the benign pathology group. The average level of CD3+, CD4+, CD8+ and NK+ cells decreased as the disease progressed. HER2+ and Luminal B HER2+ tumors had the lowest counts of cell subsets. HPV breast cancer patients had elevated counts of cellular subsets.
Determining level changes in cellular subsets in breast cancer patients is a useful tool to evaluate treatment response.
检测乳腺癌患者中人类乳头瘤病毒（HPV）的存在情况并对细胞免疫反应进行特征分析。
共纳入74名女性，其中48份样本来自被诊断为乳腺癌的患者，26份样本来自患有乳腺良性病变的患者。基于肿瘤切片的免疫组化报告进行分子亚型分类。使用INNO-LIPA HPV基因分型额外检测试剂盒（Innogenetics，根特，比利时）对新鲜乳腺活检组织进行HPV基因组检测和基因分型。通过流式细胞术检测乳腺癌患者和乳腺良性病变患者外周血样本中CD3⁺、CD4⁺、CD8⁺和自然杀伤（NK）⁺细胞水平。
管腔A型是最常见的乳腺癌分子亚型（33.33%）。在25%的乳腺癌患者中检测到HPV，在研究人群中18型是最常见的基因型。与乳腺良性病变患者相比，乳腺癌患者中CD3⁺、CD4⁺和CD8⁺亚群的平均值降低，CD8⁺值存在统计学显著差异（P = 0.048）。NK⁺细胞的平均值在乳腺良性病变组中升高。随着疾病进展，CD3⁺、CD4⁺、CD8⁺和NK⁺细胞的平均水平降低。HER2⁺和管腔B型HER2⁺肿瘤的细胞亚群计数最低。HPV阳性的乳腺癌患者细胞亚群计数升高。
确定乳腺癌患者细胞亚群的水平变化是评估治疗反应的有用工具。
