Molecular Immunology Unit for Infectious Diseases, Department of Microbiology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Department of Chemistry, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Int J Immunopathol Pharmacol. 2020 Jan-Dec;34:2058738420966822. doi: 10.1177/2058738420966822.
Several subsets of regulatory CD4+ T cells (CD4+ Tregs) have been described in peripheral blood and tumor microenvironment of breast cancer (BC) patients and may play a role in the progression of BC. High-risk human papilloma virus (HR-HPV) has a causal role in cervical, head, and neck tumors but the role of HR-HPV in evoking neoplasia in BC is still unclear. In this study we assessed the prevalence of CD4+CD25+ FOXP3+ regulatory T cells (CD4+Tregs) and CD3+ CD8+ T cells by flow cytometry in peripheral blood from a total of 55 Egyptian women, including 20 treatment-naïve BC, 15 with breast benign lesions (BBL), and 20 healthy volunteers (HV). HR-HPV genotypes type 16, 18, and 31 were investigated in breast tissue from all BC and BBL patients using Real-Time PCR. HR-HPV was detected in 4/20 (20%) and 0/15 (0%) BC and BBL patients respectively. The frequency of CD4+ Tregs was significantly higher in BC compared to BBL and HV, ( < 0.001). In addition, we observed a significantly higher frequency of CD3+ CD8+ T cells in peripheral blood of patients with late stage III BC compared to early stage I and II BC ( = 0.011). However, there was no significant association between the ratio of CD8+ T cell to CD4+ Tregs frequencies and the expression of Estrogen Receptor (ER), Progesterone Receptor (PR), and Human Epidermal Growth Factor Receptor 2 (HER2). These results lead us to postulate that the association between the frequency of CD4+ Tregs and CD8+ T cells in the peripheral blood may be a prognostic or predictive parameter in Egyptian women with BC. In addition, HR-HPV infection may be implicated in the development of some types of BC in Egyptian women.
几种调节性 CD4+T 细胞（CD4+Tregs）亚群已在乳腺癌（BC）患者的外周血和肿瘤微环境中被描述，并可能在 BC 的进展中发挥作用。高危型人乳头瘤病毒（HR-HPV）在宫颈癌、头颈部肿瘤中起因果作用，但 HR-HPV 在引发 BC 中的肿瘤形成作用尚不清楚。在这项研究中，我们通过流式细胞术评估了总共 55 名埃及女性外周血中 CD4+CD25+FOXP3+调节性 T 细胞（CD4+Tregs）和 CD3+CD8+T 细胞的患病率，包括 20 名未经治疗的 BC 患者、15 名乳腺良性病变（BBL）患者和 20 名健康志愿者（HV）。使用实时 PCR 检测所有 BC 和 BBL 患者乳腺组织中的 HR-HPV 基因型 16、18 和 31。在 20 名 BC 和 BBL 患者中分别有 4/20（20%）和 0/15（0%）检测到 HR-HPV。与 BBL 和 HV 相比，BC 患者的 CD4+Tregs 频率显着升高（ < 0.001）。此外，我们观察到晚期 III 期 BC 患者外周血中 CD3+CD8+T 细胞的频率明显高于早期 I 期和 II 期 BC（ = 0.011）。然而，CD8+T 细胞与 CD4+Tregs 频率的比值与雌激素受体（ER）、孕激素受体（PR）和人表皮生长因子受体 2（HER2）的表达之间无显着关联。这些结果使我们假设，外周血中 CD4+Tregs 和 CD8+T 细胞的频率之间的关联可能是埃及 BC 女性的预后或预测参数。此外，HR-HPV 感染可能与埃及女性某些类型的 BC 的发生有关。
