Department of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
Environ Sci Pollut Res Int. 2019 Oct;26(28):29085-29094. doi: 10.1007/s11356-019-06110-0. Epub 2019 Aug 7.
The purpose of this study was therefore to investigate the effects of radiotherapy on the kidney and the potential use of agents such as N-acetylcysteine (NAC) in developing a future therapeutic protocol for radiation-induced nephrotoxicity at the histopathological and biochemical levels. Our study consisted of three groups: control (oral saline solution only; group 1), irradiation (IR; group 2), and NAC + IR (group 3). The irradiation groups received a single dose of whole-body 6-Gy x-irradiation. The NAC group received 300 mg/kg by the oral route for 7 days, from 5 days before irradiation to 2 days after. All subjects were sacrificed under anesthesia 2 days after irradiation. IR increased tubular necrosis scores (TNS), MDA, and caspase-3 expression, while reducing renal tissue GSH levels. We also observed dilation in renal corpuscles and tubules. Capillary congestion was present in the intertubular spaces. NAC reduced the levels of TNS, MDA, and caspase-3 expression, but increased the levels of renal tissue GSH. ROS-scavenging antioxidants may represent a promising means of preventing renal injury in patients undergoing radiotherapy.
本研究旨在探讨放疗对肾脏的影响，以及在组织病理学和生化水平上开发针对放射性肾毒性的未来治疗方案时使用 N-乙酰半胱氨酸 (NAC) 等药物的潜力。我们的研究包括三组：对照组（仅口服生理盐水；第 1 组）、照射组（IR；第 2 组）和 NAC+IR 组（第 3 组）。照射组接受单次全身 6-Gy X 射线照射。NAC 组在照射前 5 天至照射后 2 天通过口服给予 300mg/kg 的 NAC。所有动物均在照射后 2 天麻醉下处死。IR 增加了肾小管坏死评分（TNS）、MDA 和 caspase-3 的表达，同时降低了肾组织 GSH 水平。我们还观察到肾小体和肾小管扩张。肾小管间空间存在毛细血管充血。NAC 降低了 TNS、MDA 和 caspase-3 的表达水平，但增加了肾组织 GSH 的水平。ROS 清除抗氧化剂可能是预防接受放疗的患者肾损伤的一种有前途的方法。
