a College of Life Science , Xinjiang Normal University , Urumqi , Xinjiang , China.
b Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology , Xinjiang University , Urumqi , Xinjiang , China.
Hum Vaccin Immunother. 2019;15(3):611-619. doi: 10.1080/21645515.2018.1547604. Epub 2018 Dec 20.
We previously found that Pleurotus ferulae polysaccharides (PFPS) improved the maturation and function of dendritic cells (DCs). In this study, we investigated the effects of PFPS on the antitumor efficacy of therapeutic human papillomavirus (HPV) DC-based vaccine. PFPS stimulated DCs pulsed with HPV E6/E7 peptides were used to treat tumor mice on day 5 & 12 (HPV + PFPS-DCs early) and day 12 & 19 (HPV + PFPS-DCs late) after TC-1 cell injection. Compared to control group, both HPV + PFPS-DCs early and HPV + PFPS-DCs late strategies significantly inhibited tumor growth, which was significantly correlated with the increased activation status of both CD4 and CD8 T cells, the decreased frequencies of myeloid-derived suppressor cells, and the induction of HPV-specific CD8 T cell responses. The survival of tumor mice was also greatly improved by HPV + PFPS-DCs early. Moreover, HPV + PFPS-DCs early completely inhibited the growth of second challenged TC-1 cells in tumor free mice. The results showed that PFPS improved the antitumor efficacy of therapeutic HPV DC-based vaccine, suggesting that PFPS might be a potential adjuvant for DC-based vaccines. This study provides a potential strategy for developing the therapeutic DC-based vaccine against cervical cancer.
我们之前发现，阿魏蘑多糖（PFPS）可改善树突状细胞（DC）的成熟和功能。在这项研究中，我们研究了 PFPS 对治疗性 HPV 树突状细胞疫苗抗肿瘤功效的影响。用 HPV E6/E7 肽脉冲刺激的 PFPS 刺激的 DC 用于在 TC-1 细胞注射后第 5 天和第 12 天（HPV + PFPS-DCs 早期）以及第 12 天和第 19 天（HPV + PFPS-DCs 晚期）治疗肿瘤小鼠。与对照组相比，HPV + PFPS-DCs 早期和 HPV + PFPS-DCs 晚期策略均显著抑制肿瘤生长，这与 CD4 和 CD8 T 细胞的激活状态增加、髓系来源的抑制细胞频率降低以及 HPV 特异性 CD8 T 细胞反应的诱导显著相关。HPV + PFPS-DCs 早期还大大提高了肿瘤小鼠的存活率。此外，HPV + PFPS-DCs 早期完全抑制了无肿瘤小鼠中第二次 TC-1 细胞挑战的生长。结果表明，PFPS 提高了治疗性 HPV 树突状细胞疫苗的抗肿瘤功效，提示 PFPS 可能是树突状细胞疫苗的潜在佐剂。这项研究为开发针对宫颈癌的治疗性 DC 疫苗提供了一种潜在策略。
