Li Jinyu, Yuan Pengfei, Wang Xinhui, Aipire Adila, Li Minjing, Yang Jie, Tao Hong, Ying Tianlei, Fu Changshuang, Wei Xianxian, Zhang Fuchun, Li Jinyao
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Food Funct. 2017 May 24;8(5):1905-1914. doi: 10.1039/c7fo00227k.
Pleurotus ferulae is an edible mushroom and has been used in Uygur medicine for a long time. In this study, we purified polysaccharides from P. ferulae (PFPS) and investigated its structural characteristics. We obtained a homogeneous PFPS with a molecular weight of around 1600 kDa and prominent characteristic polysaccharide groups, which mainly contained glucose (97%), followed by mannose and galactose (3%). Both H and C NMR spectra indicated that PFPS contained both α- and β-anomeric configurations. Atomic force microscopy and Congo red-staining data further suggested that PFPS belonged to a linear branched structure that existed in flexible single chains at low concentrations and could form aggregates such as a triple-helical structure at high concentrations. Moreover, PFPS promoted the maturation of dendritic cells through a TLR4 mediated signaling pathway, which is characterized by the increased expressions of CD40, CD86, IL-12 and TNF-α and the decreased endocytosis. The results suggest that PFPS has immunoregulatory activities.
阿魏侧耳是一种食用菌，长期以来一直被用于维吾尔医学。在本研究中，我们从阿魏侧耳中纯化出多糖（PFPS）并研究了其结构特征。我们获得了一种分子量约为1600 kDa的均一PFPS以及显著的特征多糖基团，其主要含有葡萄糖（97%），其次是甘露糖和半乳糖（3%）。氢谱和碳谱均表明PFPS同时含有α-和β-端基异构体构型。原子力显微镜和刚果红染色数据进一步表明，PFPS属于线性支链结构，在低浓度下以柔性单链形式存在，在高浓度下可形成诸如三螺旋结构的聚集体。此外，PFPS通过TLR4介导的信号通路促进树突状细胞成熟，其特征为CD40、CD86、IL-12和TNF-α表达增加以及内吞作用降低。结果表明PFPS具有免疫调节活性。
