Suido H, Zambon J J, Mashimo P A, Dunford R, Genco R J
Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
J Dent Res. 1988 Aug;67(8):1070-4. doi: 10.1177/00220345880670080201.
Bacteroides gingivalis is a Gram-negative micro-organism implicated in the pathogenesis of adult periodontitis and producing relatively large amounts of specific enzymes. In the present study, subgingival samples taken from adults with moderate periodontitis were examined for the presence and relative amounts of enzymatic activity toward certain substrates. Enzyme levels were then correlated with clinical periodontal indices and microbiological analysis of subgingival plaque, including darkfield microscopy for bacterial morphotypes and immunofluorescence microscopy for B. gingivalis and Bacteroides intermedius. The results of this study indicate a significant positive correlation between levels of enzyme capable of degrading N-benzoyl-D,L-arginine-beta-naphthylamide hydrochloride, and subgingival B. gingivalis (r = 0.55). There was a much lower correlation coefficient between this enzyme activity and subgingival B. intermedius (r = 0.26). Statistically significant (p less than 0.01) positive correlations were also demonstrated between total bacterial cell counts and levels of enzymatic activity against N-benzoyl-D,L-arginine-beta-naphthylamide hydrochloride (r = 0.76), N-carbobenzoxy-glycyl-glycyl-L-arginine-beta-naphthylamide hydrochloride (r = 0.72), and glycyl-L-proline-4-methoxy-beta-naphthylamide hydrochloride (r = 0.72), and glycyl-L-proline-4-methoxy-beta-naphthylamide hydrochloride (r = 0.69). There were significant differences in the levels of these three enzymatic activities between sites exhibiting various degrees of clinical severity of gingival inflammation and harboring various proportions of B. gingivalis. The data from this study indicate that measurement of specific enzymatic activities in subgingival samples can be useful in the diagnosis of B. gingivalis-associated periodontitis.
牙龈拟杆菌是一种革兰氏阴性微生物，与成人牙周炎的发病机制有关，并能产生相对大量的特定酶。在本研究中，对从中度牙周炎成人患者采集的龈下样本进行检测，以确定其对某些底物的酶活性的存在情况和相对含量。然后将酶水平与临床牙周指数以及龈下菌斑的微生物学分析相关联，包括用于观察细菌形态型的暗视野显微镜检查以及用于检测牙龈拟杆菌和中间普氏菌的免疫荧光显微镜检查。本研究结果表明，能够降解盐酸N-苯甲酰-D,L-精氨酸-β-萘胺的酶水平与龈下牙龈拟杆菌之间存在显著正相关（r = 0.55）。该酶活性与龈下中间普氏菌之间的相关系数则低得多（r = 0.26）。在总细菌细胞计数与针对盐酸N-苯甲酰-D,L-精氨酸-β-萘胺（r = 0.76）、盐酸N-苄氧羰基-甘氨酰-甘氨酰-L-精氨酸-β-萘胺（r = 0.72）以及盐酸甘氨酰-L-脯氨酸-4-甲氧基-β-萘胺（r = 0.72）和盐酸甘氨酰-L-脯氨酸-4-甲氧基-β-萘胺（r = 0.69）的酶活性水平之间也显示出具有统计学意义（p小于0.01）的正相关。在表现出不同程度牙龈炎症临床严重程度且含有不同比例牙龈拟杆菌的部位之间，这三种酶活性水平存在显著差异。本研究数据表明，检测龈下样本中的特定酶活性可有助于诊断与牙龈拟杆菌相关的牙周炎。
