Department of Cardiovascular Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Lianshui County People's Hospital, Huaian, China.
Biosci Rep. 2018 Dec 18;38(6). doi: 10.1042/BSR20181502. Print 2018 Dec 21.
To investigate the relationship between the miR-130a polymorphism rs731384 and coronary artery disease (CAD) and to further explore the molecular mechanism of the pathogenesis of CAD, an observational single-center study was conducted.
A total of 876 subjects were recruited in the present study. Four milliliters of venous blood was drawn after 12 h of fasting to perform biochemical assays. CAD patients and controls were distinguished by coronary angiography. Rs731384 was genotyped on the Agena MassARRAY system according to the manufacturer's user guide. Statistical analysis was conducted using SPSS 16.0 software.
The study found that the plasma levels of total cholesterol (TC) (=0.006), low-density lipoprotein cholesterol (LDL-C) (=0.030), apolipoprotein A (ApoA) (=0.038), and apolipoprotein B (ApoB) (=0.022) distributed differently in patients with various alleles. Additionally, the AA genotype of rs731384 was found to be a protective factor against CAD in a recessive model (AA:AG+GG, odds ratio (OR) = 0.408, 95% confidence interval (95% CI) = 0.171-0.973, =0.043). A significant association was found between the gene-environment interaction and CAD risk. The AA genotype along with high-density lipoprotein cholesterol (HDL-C) level ≥ 1.325 mmol/l significantly decreased the CAD risk (AA:AG+GG, OR = 0.117, 95% CI = 0.023-0.588, =0.009).
The mutant AA genotype of rs731384 seems to be a protective factor against CAD, and rs731384 plays an important role in the human metabolism of plasma lipids.
探讨 miR-130a 多态性 rs731384 与冠状动脉疾病（CAD）的关系，并进一步探讨 CAD 发病机制的分子机制，进行了一项观察性单中心研究。
本研究共招募了 876 名受试者。空腹 12 小时后抽取 4 毫升静脉血进行生化检测。通过冠状动脉造影区分 CAD 患者和对照组。根据制造商的用户指南，在 Agena MassARRAY 系统上对 rs731384 进行基因分型。使用 SPSS 16.0 软件进行统计分析。
研究发现，不同等位基因患者的总胆固醇（TC）（=0.006）、低密度脂蛋白胆固醇（LDL-C）（=0.030）、载脂蛋白 A（ApoA）（=0.038）和载脂蛋白 B（ApoB）（=0.022）的血浆水平分布不同。此外，rs731384 的 AA 基因型在隐性模型中被发现是 CAD 的保护因素（AA：AG+GG，比值比（OR）=0.408，95%置信区间（95%CI）=0.171-0.973，=0.043）。基因-环境相互作用与 CAD 风险之间存在显著关联。AA 基因型与高密度脂蛋白胆固醇（HDL-C）水平≥1.325mmol/l 相结合，显著降低 CAD 风险（AA：AG+GG，OR=0.117，95%CI=0.023-0.588，=0.009）。
rs731384 的突变 AA 基因型似乎是 CAD 的保护因素，rs731384 在人类血浆脂质代谢中起重要作用。
