Facultad de Ingeniería y Administración, Universidad Nacional de Colombia Sede Palmira, Palmira, Colombia.
Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia Sede Palmira, Palmira, Colombia.
Probiotics Antimicrob Proteins. 2019 Dec;11(4):1293-1299. doi: 10.1007/s12602-018-9481-0.
The ability of a probiotic strain (Weissella cibaria) to adhere on tissue and the effect of its topical application in nipples of lactating cows on physicochemical characteristics of milk were evaluated. An ex vivo model was used to demonstrate the adhesion capacity of W. cibaria. Tissue samples were randomly distributed in three different solutions corresponding to three treatments (a nipple bio-sealant formulation, sterile PBS solution and biomass of W. cibaria, sterile PBS solution without microorganism addition). The number of bacteria adhered in tissue was quantified and observed using electron microscopy. Additionally, a bio-sealant prepared with W. cibaria was topically applied to nipples of dairy cows. Milk samples were taken every 7 days for 60 days. Two controls were used. California mastitis test (CMT), somatic cell count, electrical conductivity, pH, density, and acidity were evaluated. The adhesion capacity of W. cibaria strain to epithelial cells of bovine teat tissue samples was demonstrated. When the strain was added as a bio-sealant, the adhesion capacity of W. cibaria was 80.44%. The response variables did not show significant differences among treatments; these results indicate the safety of the topical application of W. cibaria on the bovine mammary gland. In this study, a new safe way of administering probiotic microorganisms in nipples of lactating cows was demonstrated. W. cibaria adheres to the bovine mammary tissue and can be topically applied in nipples of lactating cows without affecting the physicochemical characteristics of milk.
本研究评估了一种益生菌菌株（魏斯氏乳酸杆菌）在组织上的黏附能力及其在哺乳期奶牛乳头局部应用对牛奶理化特性的影响。采用离体模型来证明 W. cibaria 的黏附能力。组织样本随机分布在三种不同的溶液中，对应三种处理（乳头生物密封剂配方、无菌 PBS 溶液和 W. cibaria 的生物质、无菌 PBS 溶液，未添加微生物）。使用电子显微镜定量和观察黏附在组织中的细菌数量。此外，用 W. cibaria 制备的生物密封剂被局部应用于奶牛的乳头。在 60 天内，每隔 7 天采集一次牛奶样本。使用了两个对照。评估加利福尼亚乳房炎测试（CMT）、体细胞计数、电导率、pH 值、密度和酸度。证明了 W. cibaria 菌株对牛乳头组织上皮细胞的黏附能力。当该菌株作为生物密封剂添加时，W. cibaria 的黏附能力为 80.44%。处理之间的应答变量没有显示出显著差异；这些结果表明 W. cibaria 局部应用于奶牛乳腺的安全性。在这项研究中，证明了一种在哺乳期奶牛乳头中施用益生菌微生物的新安全方法。W. cibaria 黏附于牛乳腺组织，可局部应用于哺乳期奶牛的乳头，而不影响牛奶的理化特性。
