College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Anaerobe. 2012 Oct;18(5):508-15. doi: 10.1016/j.anaerobe.2012.08.001. Epub 2012 Aug 16.
Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillus plantarum were investigated in vitro to examine their ability to competitively exclude S. aureus. Various factors involved in attachment, including bacterial status and cell concentration, growth phase, competition patterns, and surface-layer protein extracts, were also investigated. Live lactobacilli in the mid-log growth phase exhibited maximum inhibitory activity when lactobacilli were pre- or co-incubated with S. aureus. However, the inhibitory activity was significantly reduced when the lactobacilli were inactivated by heating or treated with LiCl. Furthermore, both lactobacilli possessed certain cell surface properties, such as hydrophobicity, autoaggregation, and coaggregation ability. L. salivarius and L. plantarum strongly inhibited S. aureus adherence to Caco-2 cells and their inhibition activity was significantly influenced by several factors that affect adhesion inhibition.
金黄色葡萄球菌是一种机会致病菌，能够定植于人和动物的肠道，引起某些胃肠道疾病。肠病原体对宿主肠道上皮细胞的黏附对于其发病机制非常重要。在本研究中，体外研究了唾液乳杆菌和植物乳杆菌，以考察它们竞争排斥金黄色葡萄球菌的能力。还研究了参与黏附的各种因素，包括细菌状态和细胞浓度、生长阶段、竞争模式和表面层蛋白提取物。当乳杆菌与金黄色葡萄球菌预先或共同孵育时，处于对数中期生长阶段的活菌表现出最大的抑制活性。然而，当乳杆菌通过加热失活或用 LiCl 处理时，抑制活性显著降低。此外，两种乳杆菌都具有一定的细胞表面特性，如疏水性、自聚集和共聚集能力。唾液乳杆菌和植物乳杆菌强烈抑制金黄色葡萄球菌对 Caco-2 细胞的黏附，其抑制活性受到影响黏附抑制的几个因素的显著影响。
