Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2019 Feb 1;126:222-229. doi: 10.1016/j.bios.2018.10.068. Epub 2018 Nov 2.
In this work, 3,4,9,10-perylenetetracar-boxylic acid (PTCA) as luminophor was grafted on the surface of graphene oxide (PTCA-GO) directly. GO exhibited large specific surface area and excellent electrical conductivity which can immobilize large amounts of PTCA to improve the electrochemiluminescence (ECL) efficiency. Moreover, gold nanoparticles (Au NPs) were anchored on the surface of PTCA-GO to immobilize primary antibodies (Ab) via Au-NH bond and enhance the electron transport of PTCA-GO. CuS was used as a novel co-reaction accelerator in PTCA-KSO system to label secondary antibodies (Ab), which can react with the coreactant (KSO) to produce more SO. SiO nanospheres with large specific surface area were used to load a mass of CuS and Au NPs, which can directly combine with Ab and accelerate the ECL emission remarkably. Therefore, a novel sandwich-type ECL immunosensor was fabricated successfully for amyloid-β protein (Aβ) detection. Under the optimal condition, a wide detection range from 50 fg/mL to 25 ng/mL and a low detection limit of 18 fg/mL (S/N = 3) were obtained. Featuring favorable specificity, stability and reproducibility, the strategy can be a powerful analytical tool in sensitive trace detection of biomolecules in clinical analysis.
在这项工作中，将苝四羧酸（PTCA）作为发光体直接接枝在氧化石墨烯（PTCA-GO）表面。GO 具有大的比表面积和优异的导电性，可以固定大量的 PTCA 以提高电化学发光（ECL）效率。此外，金纳米粒子（Au NPs）通过 Au-NH 键锚定在 PTCA-GO 表面上，用于固定一抗（Ab）并增强 PTCA-GO 的电子传输。CuS 被用作 PTCA-KSO 体系中的新型共反应加速剂，用于标记二抗（Ab），它可以与反应物（KSO）反应生成更多的 SO。具有大比表面积的 SiO 纳米球用于负载大量的 CuS 和 Au NPs，它们可以直接与 Ab 结合并显著加速 ECL 发射。因此，成功制备了一种新型夹心型 ECL 免疫传感器用于检测淀粉样蛋白-β 蛋白（Aβ）。在最佳条件下，从 50 fg/mL 到 25 ng/mL 获得了较宽的检测范围和 18 fg/mL（S/N=3）的低检测限。该策略具有良好的特异性、稳定性和重现性，可作为临床分析中生物分子灵敏痕量检测的有力分析工具。
