测定天然和人工培养蛹虫草中的主要核苷和碱基。
Determination of the Main Nucleosides and Nucleobases in Natural and Cultured Ophiocordyceps xuefengensis.
机构信息
College of Life Science, Hunan Normal University, Changsha 410081, China.
Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China.
出版信息
Molecules. 2017 Sep 11;22(9):1530. doi: 10.3390/molecules22091530.
, a recently described species of that is associated with the larvae of (Hepialidae) in the living root or trunk of the medicinal plant , isthe largest known species and is recognized as a desirable alternative for natural . This study investigated the main nucleosides and nucleobases in natural and cultured . The contents of the nucleosides and nucleobases in the natural and cultured samples were determined by reverse phase HPLC. The highest concentration of adenosine was found in the natural fruit body and the cultured stroma, with almost no adenosine in the cadaver of . The contents of adenine, guanosine, uridine and uracil in the cultured mycelium were significantly higher than those in the natural sample. Inosine was only detected in the natural samples. Thymidine and 2-deoxyadenosine were only found in the cadaver of . Cordycepin was not detected in the five samples examined. These results suggested that the cultured mycelium and cultured stroma of might be a promising substitute for natural .
蛹草拟青霉，是与蝙蝠蛾幼虫（蝙蝠蛾科）共生在药用植物活体根或干中的一种最近被描述的物种，是已知最大的物种，被认为是天然虫草的理想替代品。本研究调查了天然和培养蛹草拟青霉中的主要核苷和碱基。通过反相高效液相色谱法测定天然和培养样品中的核苷和碱基含量。在天然子实体和培养菌核中发现了最高浓度的腺苷，而在虫草的尸体中几乎没有腺苷。在培养的菌丝体中，腺嘌呤、鸟嘌呤、尿嘧啶和尿苷的含量明显高于天然样品。肌苷仅在天然样品中检测到。胸苷和 2-脱氧腺苷仅在虫草的尸体中发现。在所检查的五个样品中均未检测到虫草素。这些结果表明，蛹草拟青霉的培养菌丝体和培养菌核可能是天然虫草的有前途替代品。
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