Department of Animal Biotechnology, Konkuk University, Seoul, Korea.
Department of Animal Science and Technology, Konkuk University, Seoul, Korea.
Microbiologyopen. 2019 Jun;8(6):e00767. doi: 10.1002/mbo3.767. Epub 2018 Nov 16.
In recent years, Bacillus spp. have garnered attention as probiotic supplements in aquafeed owing to the production of heat stable and low pH resistant spores. Herein, we isolated and characterized an autochthonous Bacillus licheniformis KCCM 43270 from the intestine of olive flounder (Paralichthys olivaceus) for supplementation in low-fishmeal extruded aquafeeds. The KCCM 43270 was screened based on amylase, protease, cellulase, and lipase as well as non-hemolytic activities. The isolate was able to grow in carboxymethyl cellulose (CMC), xylan, and soybean meal (SBM) when used as a single carbon source in the minimal nutrient M9 medium. The KCCM 43270 spores displayed complete survival in acid (pH 2.5) and bile (0.3%, w/v) for 3 hr, strong biofilm formation, and nearly 50% adhesion with intestinal mucus. The spores of the isolate also showed significant survival ability at 80, 90, 100°C for 60, 30, and 1 min, respectively. In addition, the spores in a blend of SBM complex carrier showed significant heat stability at 120°C for 5 min and under different drying conditions. Furthermore, the spores also survived the extrusion process during low-fishmeal aquafeed manufacturing, implying the potential application of B. licheniformis KCCM 43270 in aquafeed industry.
近年来，由于芽孢能够产生耐热和耐低 pH 的孢子，因此芽孢杆菌属被视为水产饲料中的益生菌补充剂。本研究从牙鲆（Paralichthys olivaceus）肠道中分离并鉴定了一株土著地衣芽孢杆菌 KCCM 43270，将其作为补充剂添加到低鱼粉膨化水产饲料中。该菌株是根据淀粉酶、蛋白酶、纤维素酶和脂肪酶以及非溶血活性进行筛选的。该分离株能够在最小营养物 M9 培养基中使用羧甲基纤维素 (CMC)、木聚糖和豆粕 (SBM) 作为单一碳源生长。KCCM 43270 孢子在酸（pH 2.5）和胆汁（0.3%，w/v）中 3 小时内完全存活，具有很强的生物膜形成能力，并且与肠粘液的黏附率接近 50%。该分离株的孢子在 80、90、100°C 下分别存活 60、30、1 分钟，存活率也很高。此外，在 SBM 复合载体的混合物中，孢子在 120°C 下 5 分钟和不同干燥条件下具有显著的热稳定性。此外，孢子还能在低鱼粉水产饲料生产过程中经受挤压过程，这意味着地衣芽孢杆菌 KCCM 43270 具有在水产饲料工业中应用的潜力。
