Bernard C C, Mitchell G F, Leydon J, Bargerbos A
Int Arch Allergy Appl Immunol. 1978;56(3):256-63. doi: 10.1159/000232029.
Experimental autoimmune orchitis (EAO) was induced in inbred strains of mice by injection of mouse testis homogenate (MTH) in Freund's complete adjuvant with pertussis vaccine. Although all mice injected with MTH and adjuvants developed signs of EAO, there were marked strain variations in susceptibility to EAO suggesting that genetic factors may be involved in the response to antigen or to adjuvants. Studies in hypothymic BALB/c. nu/nu mice indicated that a source of T cell was required for induction of EAO. Thus BALB/c. nu/nu mice were not able to develop EAO, despite adequate orchitogenic challenge; reconstitution of nu/nu mice with syngeneic thymocytes completely restored the capacity of such mice to develop orchitis. Transfer of EAO was effected in nu/nu mice with lymphoid cells from appropriately immunized donors but not with immune serum. However, both T cells and antibodies may be necessary in the effector stages of the disease since the capacity of lymphoid cells to transfer EAO was only partially inhibited by anti-Thy-1.2 treatment.
通过在弗氏完全佐剂中加入百日咳疫苗注射小鼠睾丸匀浆（MTH），在近交系小鼠中诱导实验性自身免疫性睾丸炎（EAO）。尽管所有注射MTH和佐剂的小鼠都出现了EAO症状，但对EAO的易感性存在明显的品系差异，这表明遗传因素可能参与了对抗原或佐剂的反应。对无胸腺的BALB/c.nu/nu小鼠的研究表明，诱导EAO需要T细胞来源。因此，尽管有足够的致睾丸炎刺激，BALB/c.nu/nu小鼠仍无法发生EAO；用同基因胸腺细胞重建nu/nu小鼠完全恢复了此类小鼠发生睾丸炎的能力。用来自适当免疫供体的淋巴细胞在nu/nu小鼠中实现了EAO的转移，但用免疫血清则不能。然而，在疾病的效应阶段，T细胞和抗体可能都是必需的，因为抗Thy-1.2处理仅部分抑制了淋巴细胞转移EAO的能力。
