Department of Veterinary Public Health and Forensic Medicine, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic; Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany.
Chemosphere. 2019 Feb;217:516-521. doi: 10.1016/j.chemosphere.2018.10.208. Epub 2018 Nov 13.
Now-a-days, the occurrence of antidepressant residues in surface waters has become a major concern. Amitriptyline (AMI) has been described to treat depression and other disorders for decades. However, little is known about its effect on non-target organisms. The aim of this study was to assess the potential impact of AMI on the mRNA expression of antioxidant and detoxification enzymes during the early embryonic development of zebrafish (Danio rerio). Fertilized D. rerio embryos were exposed to AMI at concentrations of 300 ng/L and 30 μg/L and sampled 24, 48, 96, and 144 h post fertilization (hpf) to assess the mRNA expressions of cytochrome P450 1A1, glutathione-S-transferase, glutathione peroxidase, superoxide dismutase, and catalase. The time courses of the mRNA expressions of antioxidant and detoxification enzymes revealed characteristic changes during embryonic development causing generally transient changes post hatching; however, AMI did not cause any significant impact except in the case of CAT after 144 h, which was significantly upregulated by the AMI concentration of 30 μg/L. The results suggest that the antidepressant AMI causes only moderate to minor impacts on antioxidant and detoxification enzymes during early embryonic development of the non-target organism D. rerio and that CAT is the only biomarker affected by AMI.
如今，抗抑郁药残留出现在地表水中已经成为一个主要问题。阿米替林（AMI）已被描述为数十年来治疗抑郁症和其他疾病的药物。然而，人们对其对非靶标生物的影响知之甚少。本研究旨在评估 AMI 对斑马鱼（Danio rerio）早期胚胎发育过程中抗氧化和解毒酶的 mRNA 表达的潜在影响。受精后的斑马鱼胚胎分别暴露于 300ng/L 和 30μg/L 的 AMI 中，并在受精后 24、48、96 和 144 小时取样，以评估细胞色素 P450 1A1、谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶的 mRNA 表达。抗氧化和解毒酶的 mRNA 表达的时程在胚胎发育过程中呈现出特征性的变化，导致孵化后通常出现短暂的变化；然而，除了在 144 小时时 CAT 之外，AMI 并没有引起任何显著的影响，30μg/L 的 AMI 浓度显著上调了 CAT。研究结果表明，抗抑郁药 AMI 仅对非靶标生物斑马鱼的早期胚胎发育过程中的抗氧化和解毒酶产生中度至较小的影响，并且 CAT 是唯一受到 AMI 影响的生物标志物。
