Department of Horticulture, Citrus Research and Education Center-University of Florida/IFAS, Lake Alfred, FL, USA.
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Nat Prod Res. 2020 Mar;34(6):898-902. doi: 10.1080/14786419.2018.1508145. Epub 2018 Nov 16.
This study evaluated the roles of zeatin (2 mg/L) on direct organogenesis, phytochemical compounds, antioxidant activity, and cytotoxic activity in regenerated shoots of red cabbage. The results revealed that the extract of explant treated by 2 mg/L zeatin gives the highest content of total phenolics (5.18 mg gallic acid equivalent/g dry weight) and flavonoids (1.52 mg rutin equivalent/g dry weight). Moreover, HPLC and GC-MS analyses indicated that various bioactive compounds in red cabbage are significantly enhanced with increasing zeatin concentration. Besides that, antioxidant activity test showed that shooting culture using 2 mg/L zeatin displayed higher antioxidant activity in all assays (DPPH, FRAP and ABTS) compared to control with respective values of 68.12%, 73.28%, and 54.1%, respectively. Finally, the cytotoxic properties illustrated that the extracts of red cabbage explant treated by 2 mg/L zeatin exhibited the strongest cytotoxic effect towards cancer cells compared to control.
本研究评估了玉米素（2mg/L）在紫菜头直接器官发生、植物化学化合物、抗氧化活性和再生芽的细胞毒性中的作用。结果表明，用 2mg/L 玉米素处理的外植体提取物具有最高的总酚含量（5.18mg 没食子酸当量/g 干重）和类黄酮含量（1.52mg 芦丁当量/g 干重）。此外，HPLC 和 GC-MS 分析表明，随着玉米素浓度的增加，紫菜头中的各种生物活性化合物显著增强。此外，抗氧化活性测试表明，与对照相比，使用 2mg/L 玉米素的芽培养在所有测定中（DPPH、FRAP 和 ABTS）均显示出更高的抗氧化活性，分别为 68.12%、73.28%和 54.1%。最后，细胞毒性特性表明，与对照相比，用 2mg/L 玉米素处理的紫菜头外植体提取物对癌细胞表现出最强的细胞毒性作用。
