College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China.
College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Carbohydr Polym. 2019 Feb 1;205:236-243. doi: 10.1016/j.carbpol.2018.10.045. Epub 2018 Oct 16.
The co-solvent prepared from glycerol and hydrochloric acid (HCl) was used for producing low molecular weight chitin from lobster shell. Different reaction temperature, time and acid concentration were studied. The results suggested that the effect of reaction temperature and acid concentration on molecular weight of chitin is most remarkable. The physicochemical properties of chitins were intact during co-solvent treatment. At least 5% HCl was necessary for demineralization and deproteinization. Low molecular weight chitin could be obtained by increasing reaction temperature, acid concentration and prolonging time. The optimized condition for production low molecular weight chitin was at 120 °C for 2 h with acid concentration of 7% (55 kDa) or at 150 °C for 2 h with acid concentration of 5% (57 kDa). This method provides a fast strategy to obtain low molecular weight chitin with high purity from lobster shell.
由甘油和盐酸（HCl）制成的共溶剂用于从龙虾壳中生产低分子量壳聚糖。研究了不同的反应温度、时间和酸浓度。结果表明，反应温度和酸浓度对壳聚糖分子量的影响最为显著。共溶剂处理过程中壳聚糖的物理化学性质保持完整。至少需要 5%的 HCl 进行脱矿质和脱蛋白。通过提高反应温度、酸浓度和延长时间，可以获得低分子量壳聚糖。生产低分子量壳聚糖的最佳条件是在 120°C 下反应 2 小时，酸浓度为 7%（55kDa），或在 150°C 下反应 2 小时，酸浓度为 5%（57kDa）。该方法为从龙虾壳中快速获得高纯度低分子量壳聚糖提供了一种策略。
