Key Laboratory of Green Process Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Zhongguancun, Haidian District, Beijing, 100190, China; Beijing BW Techtextile Co., Ltd, Floor 11, Building 2, Block 26, Financial Street (Changan Center), Shijingshan District, Beijing, 100043, China.
Key Laboratory of Green Process Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Zhongguancun, Haidian District, Beijing, 100190, China; University of Chinese Academy of Sciences, Yuquan Rd, Shijingshan District, Beijing, 100049, China.
Carbohydr Polym. 2018 Jun 15;190:148-155. doi: 10.1016/j.carbpol.2018.02.019. Epub 2018 Feb 24.
Low molecular weight (LMW) chitin exhibits antimicrobial activity, and its preparation generally employs high molecular weight chitin as raw material. Here we use raw crab shell as material to isolate LMW-chitin. The raw crab shell was treated with hydrochloric acid under different conditions, and the demineralization and deproteinization of acid to the raw and demineralized crab shell were examined. Under conditions of 3-5 wt% acid, 110 °C, and 180-600 min, the obtained LMW-chitin owns 92% purity and 53-80 kDa molecular weight. The method provides feasible one-step base-free strategy to directly obtain LMW-chitin from raw crab shell, and could be potentially extended to other chitin sources.
低相对分子质量（LMW）壳聚糖具有抗菌活性，其制备通常以高分子量壳聚糖为原料。本研究以生蟹壳为原料分离 LMW-壳聚糖。用不同条件的盐酸处理生蟹壳，考察酸对生蟹壳和脱钙蟹壳的脱矿质和脱蛋白作用。在 3-5wt%酸、110°C 和 180-600min 的条件下，得到的 LMW-壳聚糖纯度为 92%，相对分子质量为 53-80kDa。该方法提供了一种可行的无碱一步法策略，可直接从生蟹壳中获得 LMW-壳聚糖，有望扩展到其他壳聚糖来源。
