利用粪产碱杆菌从桔皮废弃物中可持续生物合成出卡拉胶：一种系统建模方法。
Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis: A systematically modeled approach.
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China.
出版信息
Carbohydr Polym. 2019 Feb 1;205:626-635. doi: 10.1016/j.carbpol.2018.10.047. Epub 2018 Oct 25.
This study presents an engineered approach for sustainable biosynthesis of curdlan by Alcaligenes faecalis using orange peels. To confirm the substrate suitability a four step study was organized. Firstly, drying of substrate was carried within temperature range of 60-120 °C, along with the application of moisture diffusion control model. Secondly, fermentation medium was obtained via saccharification and detoxification, releasing highest sugar at 72.34 g/L with phenolics removal of 95-98%. Thirdly, curdlan fermentation was conducted in detoxified orange peel hydrolysate followed by optimization of batch culture fermentation via kinetic modeling using Logistic and Luedeking-Piret equations. In 5 L bioreactor, highest specific growth rate (μ = 0.233/h), highest curdlan production (P = 23.24 g/L) and growth associated rate constant (α = 3.403) were achieved. Moreover, the total sugar consumption and conversion rates were 83.27% and 53.20%. Lastly, characterization techniques such as FTIR, NMR, XRD, TGA, HPGPC and EDS were applied to biosynthesized curdlan for qualitative validation.
本研究提出了一种利用桔皮通过粪产碱杆菌可持续生物合成可得然胶的工程方法。为了确认底物的适用性，我们组织了四步研究。首先，在 60-120°C 的温度范围内进行了底物干燥，并应用了水分扩散控制模型。其次，通过糖化和解毒获得发酵培养基，在 72.34g/L 下释放出最高的糖，酚类去除率为 95-98%。第三，在解毒后的桔皮水解物中进行可得然胶发酵，然后通过使用Logistic 和 Luedeking-Piret 方程的动力学建模对分批培养发酵进行优化。在 5L 生物反应器中，实现了最高的比生长速率（μ=0.233/h）、最高的可得然胶产量（P=23.24g/L）和与生长相关的速率常数（α=3.403）。此外，总糖消耗和转化率分别为 83.27%和 53.20%。最后，应用 FTIR、NMR、XRD、TGA、HPGPC 和 EDS 等技术对生物合成的可得然胶进行定性验证。
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