UCLouvain, Université catholique de Louvain, Louvain Drug Research Institute, Pharmacognosy Research Group, Avenue E. Mounier, B1 72.03, B-1200 Brussels, Belgium.
UCLouvain, Université catholique de Louvain, Louvain Drug Research Institute, Pharmacognosy Research Group, Avenue E. Mounier, B1 72.03, B-1200 Brussels, Belgium.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jan 1;1104:109-118. doi: 10.1016/j.jchromb.2018.11.003. Epub 2018 Nov 4.
The aim of this study is to develop validated methods for the extraction and quantification of antimalarial triterpene esters from Keetia leucantha and from plasma samples. These compounds, showing in vitro and in vivo antiplasmodial activities, were optimally extracted from Keetia leucantha twigs using ultrasounds with dichloromethane and from plasma using protein precipitation with acetonitrile. We then developed and validated HPLC-UV quantification methods, which proved to be selective, accurate, linear, true and precise, both in plant and plasma samples for the eight triterpenic esters in mixture. Based on the total error concept as decision criteria, the validated dosage ranges of the triterpene esters mixture were set between 14.68 and 73.37 μg/mL in plants and 15.90 and 106.01 μg/mL in plasma injected solutions, corresponding to 7.95 and 53.01 μg/mL in plasma. These reliable methods were used to determine effectively triterpene esters content in collected samples, that seems highly variable in plant extracts, and will be helpful to further investigate pharmacokinetics parameters of these interesting bioactive compounds.
本研究旨在开发从 Keetia leucantha 和血浆样品中提取和定量抗疟三萜酯的经过验证的方法。这些化合物在体外和体内均具有抗疟活性，使用二氯甲烷通过超声从 Keetia leucantha 小枝中最佳提取，使用乙腈进行蛋白质沉淀从血浆中提取。然后，我们开发并验证了 HPLC-UV 定量方法，该方法在植物和血浆样品中的混合物中的八种三萜酯均表现出选择性、准确性、线性、真实性和精密度。基于总误差概念作为决策标准，验证的三萜酯混合物剂量范围在植物中的 14.68 至 73.37 μg/mL 之间，在血浆中的 15.90 至 106.01 μg/mL 之间，相当于血浆中的 7.95 和 53.01 μg/mL。这些可靠的方法可用于有效地确定收集样品中的三萜酯含量，这些含量在植物提取物中差异很大，这将有助于进一步研究这些有趣的生物活性化合物的药代动力学参数。
