纳米金刚石与上转换纳米粒子的结合用于生物成像和药物传递。

Nanodiamonds conjugated upconversion nanoparticles for bio-imaging and drug delivery.

机构信息

Henan Key Laboratory of Diamond Optoelectronic Materials and Devices, School of Physics and Engineering, Zhengzhou University, Zhengzhou 450001, China.

出版信息

J Colloid Interface Sci. 2019 Mar 1;537:316-324. doi: 10.1016/j.jcis.2018.11.028. Epub 2018 Nov 10.

DOI:10.1016/j.jcis.2018.11.028

PMID:30448652

Abstract

A multifunctional nanoplatform with simultaneous imaging and therapeutic capacities has been fabricated by covalently conjugating nanodiamonds (NDs) with upconversion NaYF:Yb,Er nanoparticles (UCNPs). Green emission can be observed for the UCNP-NDs composites under 980 nm excitation, and in vitro imaging was carried out towards HeLa cells. The UCNP-NDs also shows good drug storage capability toward anticancer drug doxorubicin (Dox) and exhibits significant pH-dependent drug-release behavior. Dox-loaded UCNP-NDs shows higher therapy efficiency towards Hela cells than free Dox when the equivalent concentration of the Dox is more than 10 μg/mL. Thus, a favorable strategy may be provided to decrease the side effects of Dox, minimize drug dose, and improve its efficacy. These findings highlight the fascinating features of UCNP-NDs nanocomposites as upconversion fluorescence imaging contrast agents and doxorubicin loading carrier. In addition, this work may also provide a novel strategy for the design of multifunctional nanoplatforms.

摘要

通过将纳米金刚石（NDs）与上转换 NaYF:Yb,Er 纳米粒子（UCNPs）共价键合，制备了具有同时成像和治疗能力的多功能纳米平台。在 980nm 激发下，UCNP-NDs 复合材料可以观察到绿色发射，并且对 HeLa 细胞进行了体外成像。UCNP-NDs 对抗癌药物阿霉素（Dox）也表现出良好的药物储存能力，并表现出显著的 pH 依赖性药物释放行为。当 Dox 的等效浓度大于 10μg/mL 时，负载 Dox 的 UCNP-NDs 对 Hela 细胞的治疗效率高于游离 Dox。因此，可能提供了一种降低 Dox 副作用、减少药物剂量和提高疗效的有利策略。这些发现突出了 UCNP-NDs 纳米复合材料作为上转换荧光成像造影剂和阿霉素负载载体的迷人特性。此外，这项工作还可能为多功能纳米平台的设计提供一种新策略。