蛋黄免疫球蛋白补充剂可预防黄曲霉毒素 B 诱导的大鼠肝氧化损伤和遗传毒性。
Egg Yolk Immunoglobulin Supplementation Prevents Rat Liver from Aflatoxin B-Induced Oxidative Damage and Genotoxicity.
机构信息
Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food Science , South China Agricultural University , Guangzhou 510642 , China.
Key Laboratory of Environment and Female Reproductive Health/Public Health Laboratory Sciences and Toxicology, West China School of Public Health , Sichuan University , Chengdu , China.
出版信息
J Agric Food Chem. 2018 Dec 19;66(50):13260-13267. doi: 10.1021/acs.jafc.8b04659. Epub 2018 Dec 4.
Egg yolk immunoglobulins (IgY), as nutraceutical supplement for therapeutic or prophylactic intervention, have been extensively studied. The effects of IgY on small molecular toxin-induced toxicity in animals are unclear. In the present study, the protection of highly purified and specific anti-AFB IgY against AFB-induced genotoxicity and oxidative damage on the rat liver model were investigated. Our results revealed that AFB induced significant oxidative damage markers, as well as AFB-induced protein expression in antioxidant, pro- and antiapoptosis processes in rat liver. These effects could be significantly inhibited by cogavage with anti-AFB IgY in a dose-dependent manner. However, anti-AFB IgY did not significantly induce hepatic CAT and SOD1. To explore mechanisms, metabolite experiments were established to evaluate the influence of anti-AFB IgY on the absorption of AFB in rats. Middle and high doses of anti-AFB IgY reduced hepatic AFB-DNA adducts by 43.3% and 52.9%, AFB- N-guanine urinary adducts by 19.6% and 34.4%, and AFB-albumin adducts by 10.5% and 21.1%, respectively. The feces of high dose anti-AFB IgY cogavaged rats contained approximately 2-fold higher AFB equivalents at 3-6 h after ingestion than AFB group feces, indicating IgY inhibited AFB uptake. These results had provided insight that anti-AFB IgY could prevent animal organs from damage caused by AFB and will be beneficial for the application of detoxification antibody as a supplement in food.
蛋黄免疫球蛋白（IgY）作为治疗或预防干预的营养补充剂，已经得到了广泛的研究。然而，IgY 对小分子毒素诱导的动物毒性的影响尚不清楚。在本研究中，研究了高度纯化和特异的抗 AFB IgY 对 AFB 诱导的大鼠肝脏模型遗传毒性和氧化损伤的保护作用。结果表明，AFB 诱导了显著的氧化损伤标志物，以及抗氧化、促凋亡和抗凋亡过程中 AFB 诱导的蛋白质表达。这些效应可以通过与抗 AFB IgY 共孵育以剂量依赖的方式显著抑制。然而，抗 AFB IgY 对肝 CAT 和 SOD1 的诱导作用不明显。为了探讨机制，建立了代谢物实验来评估抗 AFB IgY 对大鼠 AFB 吸收的影响。中高剂量的抗 AFB IgY 使肝 AFB-DNA 加合物减少了 43.3%和 52.9%，AFB-N-鸟嘌呤尿加合物减少了 19.6%和 34.4%，AFB-白蛋白加合物减少了 10.5%和 21.1%。高剂量抗 AFB IgY 共孵育大鼠的粪便在摄入后 3-6 小时内含有约 2 倍更高的 AFB 当量，表明 IgY 抑制了 AFB 的摄取。这些结果表明，抗 AFB IgY 可以防止 AFB 对动物器官造成损伤，这将有助于解毒抗体作为食品补充剂的应用。
Zotero 插件