大肠杆菌中通过基因转换进行的DNA双链缺口修复
Double-stranded gap repair of DNA by gene conversion in Escherichia coli.
作者信息
Kobayashi I, Takahashi N
机构信息
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Genetics. 1988 Aug;119(4):751-7. doi: 10.1093/genetics/119.4.751.
Abstract
We demonstrated repair of a double-stranded DNA gap through gene conversion by a homologous DNA sequence in Escherichia coli. We made a double-stranded gap in one of the two regions of homology in an inverted orientation on a plasmid DNA molecule and introduced it into an E. coli strain which has the RecE system of recombination (genotype; sbcA23 recB21 recC22). We detected repair products by genetic selection. The repair products were those expected by the double-strand-gap repair model. Gene conversion was frequently accompanied by crossing over of the flanking sequences as in eukaryotes. This double-strand gap repair mechanism can explain plasmid recombination in the absence of an artificial double-stranded break reported in a companion study by Yamamoto et al.
摘要
我们证明了在大肠杆菌中通过同源DNA序列的基因转换来修复双链DNA缺口。我们在质粒DNA分子上以反向排列的两个同源区域之一制造了一个双链缺口，并将其引入具有RecE重组系统（基因型：sbcA23 recB21 recC22）的大肠杆菌菌株中。我们通过遗传筛选检测到了修复产物。修复产物是双链缺口修复模型所预期的那些。与真核生物一样，基因转换经常伴随着侧翼序列的交叉。这种双链缺口修复机制可以解释山本等人在一项配套研究中报道的在没有人工双链断裂情况下的质粒重组。
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