Popov Daniil V, Makhnovskii Pavel A, Kurochkina Nadia S, Lysenko Evgeny A, Vepkhvadze Tatiana F, Vinogradova Olga L
Laboratory of exercise physiology, Institute of Biomedical problems of the Russian Academy of Sciences, Moscow, Russia.
Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, Russia.
Biol Sport. 2018 Sep;35(3):277-289. doi: 10.5114/biolsport.2018.77828. Epub 2018 Aug 27.
We investigated acute exercise-induced gene expression in skeletal muscle adapted to aerobic training. Vastus lateralis muscle samples were taken in ten endurance-trained males prior to, and just after, 4 h, and 8 h after acute cycling sessions with different intensities, 70% and 50% . High-throughput RNA sequencing was applied in samples from two subjects to evaluate differentially expressed genes after intensive exercise (70% ), and then the changes in expression for selected genes were validated by quantitative PCR (qPCR). To define exercise-induced genes, we compared gene expression after acute exercise with different intensities, 70% and 50% , by qPCR. The transcriptome is dynamically changed during the first hours of recovery after intensive exercise (70% ). A computational approach revealed that the changes might be related to up- and down-regulation of the activity of transcription activators and repressors, respectively. The exercise increased expression of many genes encoding protein kinases, while genes encoding transcriptional regulators were both up- and down-regulated. Evaluation of the gene expression after exercise with different intensities revealed that some genes changed expression in an intensity-dependent manner, but others did not: the majority of genes encoding protein kinases, oxidative phosphorylation and activator protein (AP)-1-related genes significantly correlated with markers of exercise stress (power, blood lactate during exercise and post-exercise blood cortisol), while transcriptional repressors and circadian-related genes did not. Some of the changes in gene expression after exercise seemingly may be modulated by circadian rhythm.
我们研究了适应有氧训练的骨骼肌中急性运动诱导的基因表达。在10名耐力训练男性进行不同强度（70%和50%）的急性骑行训练前、训练后4小时和8小时采集股外侧肌样本。对两名受试者的样本应用高通量RNA测序来评估高强度运动（70%）后差异表达的基因，然后通过定量PCR（qPCR）验证所选基因的表达变化。为了确定运动诱导的基因，我们通过qPCR比较了不同强度（70%和50%）急性运动后的基因表达。在高强度运动（70%）后的恢复最初几小时内，转录组发生动态变化。一种计算方法表明，这些变化可能分别与转录激活因子和抑制因子活性的上调和下调有关。运动增加了许多编码蛋白激酶的基因的表达，而编码转录调节因子的基因既有上调也有下调。对不同强度运动后的基因表达评估表明，一些基因的表达以强度依赖的方式变化，但其他基因则不然：大多数编码蛋白激酶、氧化磷酸化和激活蛋白（AP）-1相关的基因与运动应激标志物（功率、运动期间的血乳酸和运动后血皮质醇）显著相关，而转录抑制因子和昼夜节律相关基因则不然。运动后基因表达的一些变化似乎可能受昼夜节律调节。
