Tunisian Olive Institute, University of Sfax, Sfax, Tunisia.
Institute for Microbiology and Biochemistry, Geisenheim University, Geisenheim, Germany.
J Appl Microbiol. 2019 Feb;126(2):489-502. doi: 10.1111/jam.14159. Epub 2018 Dec 26.
This study investigated the bacterial diversity of dead insects collected from olive tree orchards widely cultivated in Tunisia. We aimed to explore metabolic diversity, screen enzymatic activities for biotechnological applications and carry out preliminary bioassays for bio-insecticide development.
A total of 50 bacteria strains were randomly isolated from four different biotopes situated in Sfax (Tunisia). 16S rRNA gene sequencing was used to identify 24 species, and isolates were evaluated for enzymatic activity and antimicrobial potential. Nineteen of the total number of Bacillus strains have enzymatic activity compared to strains from the other genera (14/50). Our bacterial collection was evaluated for antimicrobial potential against bacterial and fungal isolates. Isolates B8-2 (Bacillus subtilis) showed strong antibacterial activities and isolates B2-3 (Bacillus licheniformis) and B10-1 (Serratia marcescens) showed the highest antifungal activity. Seventeen of the total number of isolates caused greater than 50% mortality rate of second and fourth instar larvae of Ephestia kuehniella.
The selected species from olive orchards represent a broad spectrum of antimicrobial and insecticidal activities and can be considered promising resources in biological control.
Bacteria isolated from olive pests in olive orchards were investigated for detecting their potential biotechnological applications.
本研究调查了从突尼斯广泛种植的橄榄园中采集的死虫的细菌多样性。我们旨在探索代谢多样性，筛选用于生物技术应用的酶活性，并进行生物杀虫剂开发的初步生物测定。
从位于斯法克斯（突尼斯）的四个不同生境中随机分离了 50 株细菌菌株。使用 16S rRNA 基因测序鉴定了 24 个种，并且对分离株的酶活性和抗菌潜力进行了评估。与其他属的菌株相比，总数为 19 株的芽孢杆菌菌株具有酶活性（50/50）。我们的细菌收集物针对抗细菌和真菌分离物的抗菌潜力进行了评估。分离株 B8-2（枯草芽孢杆菌）表现出很强的抗菌活性，而分离株 B2-3（地衣芽孢杆菌）和 B10-1（粘质沙雷氏菌）表现出最高的抗真菌活性。总共有 17 株分离株对第二和第四龄的Ephestia kuehniella 幼虫的死亡率超过 50％。
橄榄园中选择的物种具有广泛的抗菌和杀虫活性，可以被认为是生物防治中有前途的资源。
从橄榄园中害虫中分离出的细菌被调查以检测其潜在的生物技术应用。
