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TaCIPK10 与 TaNH2 相互作用并使其磷酸化，从而激活小麦对条锈病的防御反应。

TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust.

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.

出版信息

Plant Biotechnol J. 2019 May;17(5):956-968. doi: 10.1111/pbi.13031. Epub 2018 Dec 5.

DOI:10.1111/pbi.13031
PMID:30451367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6587807/
Abstract

Calcineurin B-like interacting protein kinase (CIPKs) has been shown to be required for biotic stress tolerance of plants in plant-pathogen interactions. However, the roles of CIPKs in immune signalling of cereal crops and an in-depth knowledge of substrates of CIPKs in response to biotic stress are under debate. In this study, we identified and cloned a CIPK homologue gene TaCIPK10 from wheat. TaCIPK10 was rapidly induced by Puccinia striiformis f. sp. tritici (Pst) inoculation and salicylic acid (SA) treatment. In vitro phosphorylation assay demonstrated that the kinase activity of TaCIPK10 is regulated by Ca and TaCBL4. Knockdown TaCIPK10 significantly reduced wheat resistance to Pst, whereas TaCIPK10 overexpression resulted in enhanced wheat resistance to Pst by the induction of defense response in different aspects, including hypersensitive cell death, ROS accumulation and pathogenesis-relative genes expression. Moreover, TaCIPK10 physically interacted with and phosphorylated TaNH2, which was homologous to AtNPR3/4. Silencing of TaNH2 in wheat resulted in enhanced susceptibility to the avirulent Pst race, CYR23, indicating its positive role in wheat resistance. Our results demonstrate that TaCIPK10 positively regulate wheat resistance to Pst as molecular links between of Ca and downstream components of defense response and TaCIPK10 interacts with and phosphorylates TaNH2 to regulate wheat resistance to Pst.

摘要

钙调磷酸酶 B 样相互作用蛋白激酶（CIPKs）已被证明在植物与病原体互作过程中对于植物的生物胁迫耐受性是必需的。然而，CIPKs 在谷类作物免疫信号中的作用以及 CIPKs 对生物胁迫的底物的深入了解仍存在争议。在本研究中，我们从小麦中鉴定并克隆了一个 CIPK 同源基因 TaCIPK10。TaCIPK10 被小麦条锈菌（Puccinia striiformis f. sp. tritici，Pst）接种和水杨酸（salicylic acid，SA）处理迅速诱导。体外磷酸化实验表明，TaCIPK10 的激酶活性受 Ca 和 TaCBL4 调节。TaCIPK10 的敲低显著降低了小麦对 Pst 的抗性，而 TaCIPK10 的过表达通过诱导防御反应的不同方面，包括过敏性细胞死亡、ROS 积累和病程相关基因表达，增强了小麦对 Pst 的抗性。此外，TaCIPK10 与 TaNH2 相互作用并磷酸化 TaNH2，TaNH2 与拟南芥 NPR3/4 同源。在小麦中沉默 TaNH2 导致对无毒 Pst 小种 CYR23 的敏感性增强，表明其在小麦抗性中起积极作用。我们的结果表明，TaCIPK10 通过与防御反应下游组分的 Ca 和分子链接正向调控小麦对 Pst 的抗性，并且 TaCIPK10 与 TaNH2 相互作用并磷酸化 TaNH2 以调控小麦对 Pst 的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e555/11386708/23976f037f1d/PBI-17-956-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e555/11386708/85743e92ca3c/PBI-17-956-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e555/11386708/48a9d03ad65e/PBI-17-956-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e555/11386708/6ed527d7bdda/PBI-17-956-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e555/11386708/23976f037f1d/PBI-17-956-g004.jpg

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2
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3
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