Yin Shuining, Wang Chenfang, Jiao Min, Li Feng, Han Qingmei, Huang Lili, Zhang Hongchang, Kang Zhensheng
State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China.
Protoplasma. 2015 Jan;252(1):103-16. doi: 10.1007/s00709-014-0659-3. Epub 2014 Jun 10.
Ca(2+) is an ubiquitous intracellular molecule which is used as a second messenger to control many physiological activities in plant cells. In the present work, the relationship between calcium localization and the hypersensitive response (HR)one of the most crucial and indispensable pathway to resist a pathogenwas studied in the wheat-wheat strip rust system using cytochemical technique. Our results show that calcium is involved in the interaction between wheat and wheat stripe rust. In the incompatible interaction associated with necrosis of host mesophyll cells, an influx of Ca(2+) from the intercellular space to the cytoplasm and finally an efflux to the intercellular space again was detected in an incompatible interaction. Calcium precipitates were also observed in mesophyll cells adjacent to necrotic cells. On the contrary, calcium flow was not significantly altered in a compatible interaction. These results suggest that calcium might induce HR as a secondary messenger in the incompatible interaction of wheat and wheat stripe rust.
钙离子是一种普遍存在的细胞内分子，作为第二信使参与调控植物细胞的多种生理活动。在本研究中，利用细胞化学技术，在小麦-小麦条锈菌体系中研究了钙定位与过敏反应（HR）（植物抵御病原菌最重要且不可或缺的途径之一）之间的关系。结果表明，钙参与了小麦与小麦条锈菌的互作过程。在与寄主叶肉细胞坏死相关的不亲和互作中，检测到钙离子从细胞间隙流入细胞质，最终又再次流出到细胞间隙。在坏死细胞相邻的叶肉细胞中也观察到了钙沉淀。相反，在亲和互作中钙流没有明显变化。这些结果表明，在小麦与小麦条锈菌的不亲和互作中，钙可能作为第二信使诱导过敏反应。
