Cai Ruihang, Maria Munawar, Qu Nan, Castillo Pablo, Zheng Jingwu
Laboratory of Plant Nematology, College of Agriculture & Biotechnology, Institute of Biotechnology, Hangzhou 310058, Zhejiang, P.R. China.
Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, 14004 Córdoba, Spain.
J Nematol. 2018;50(1):1-18. doi: 10.21307/jofnem-2018-016.
Longidorids are economically important plant-parasitic nematodes because several species are virus vectors. Populations of Paralongidorus sali and Longidorus jonesi , isolated from woody perennials of Hangzhou, Zhejiang, China, were characterized molecularly and morphologically. The morphometric data of the Chinese populations of both species were compared with other populations. The present study provided a first record of the occurrence of Paralongidorus in China coupled with description of the first-stage Juvenile and male of L. jonesi . Phylogenetic analysis based on 18S and D2-D3 expansion segments of 28S gene indicated that L. jonesi clustered with L. jonesi reported from Japan and P. sali grouped with P. bikanerensis from Iran. Considering the pathological and economic importance of this group of nematodes, the study emphasized the need of updated descriptions from accurately identified specimens, isolation of sufficient material for examination, and molecular and phylogenetic analysis for a better understanding and diagnostics of Longidorid nematodes.
长针线虫是具有经济重要性的植物寄生线虫，因为有几个物种是病毒传播媒介。从中国浙江杭州的木本多年生植物中分离出的萨氏拟长针线虫和琼斯长针线虫种群，进行了分子和形态学特征分析。将这两个物种中国种群的形态测量数据与其他种群进行了比较。本研究首次记录了拟长针线虫在中国的出现，并描述了琼斯长针线虫的第一龄幼虫和雄虫。基于28S基因的18S和D2-D3扩增片段的系统发育分析表明，琼斯长针线虫与日本报道的琼斯长针线虫聚类在一起，萨氏拟长针线虫与来自伊朗的比卡内尔拟长针线虫归为一类。考虑到这组线虫的病理学和经济重要性，该研究强调需要对准确鉴定的标本进行更新描述，分离足够的材料进行检查，并进行分子和系统发育分析，以更好地了解和诊断长针线虫。
