Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
BML Inc., Kawagoe, Saitama, Japan.
PLoS One. 2018 Nov 19;13(11):e0207134. doi: 10.1371/journal.pone.0207134. eCollection 2018.
The first outbreak in Japan of GES-5 carbapenemase-producing Pseudomonas aeruginosa occurred in a long-term care facility in 2014. To assess the spread of GES-5 producing P. aeruginosa clinical isolates in medical settings in Japan, 1,476 carbapenem-resistant P. aeruginosa isolates obtained from 2012 to 2016 were characterized. Of these 1,476 isolates, 104 (7.0%) harbored blaGES-5. Southern blotting revealed that the blaGES-5 was located on the chromosome. The isolation rates of these GES-5 producers increased significantly every year, from 2.0% (6 of 295) in 2012 to 2.8% (8 of 283) in 2013 to 5.3% (16 of 303) in 2014 to 9.7% (29 of 300) in 2015 to 15.3% (45 of 295) in 2016. Of the 104 GES-5 producers, 102 belonged to clonal complex (CC) 235, including 99 belonging to ST235 and three belonging to ST2233). Whole genome sequence analysis revealed that CC235 P. aeruginosa harboring blaGES-5 spread in a clonal manner. These results indicate that these GES-5 producing CC235 P. aeruginosa clinical isolates have spread in medical settings throughout Japan.
2014 年，日本在一家长期护理机构首次发现产 GES-5 碳青霉烯酶铜绿假单胞菌。为评估 GES-5 产铜绿假单胞菌临床分离株在日本医疗机构中的传播情况，对 2012 年至 2016 年获得的 1476 株耐碳青霉烯铜绿假单胞菌进行了特征分析。在这 1476 株分离株中，有 104 株（7.0%）携带 blaGES-5。Southern 印迹显示 blaGES-5 位于染色体上。这些 GES-5 产生者的分离率每年显著增加，从 2012 年的 2.0%（295 例中的 6 例）增加到 2013 年的 2.8%（283 例中的 8 例）、2014 年的 5.3%（303 例中的 16 例）、2015 年的 9.7%（300 例中的 29 例）和 2016 年的 15.3%（295 例中的 45 例）。在 104 株 GES-5 产生者中，有 102 株属于克隆群（CC）235，包括 99 株属于 ST235 和 3 株属于 ST2233）。全基因组序列分析显示，携带 blaGES-5 的 CC235 铜绿假单胞菌以克隆方式传播。这些结果表明，这些在医疗环境中传播的产 GES-5 CC235 铜绿假单胞菌临床分离株。
