日本由于 ST235 克隆扩张导致产 GES-5 碳青霉烯酶铜绿假单胞菌临床分离株的传播。
Spread of GES-5 carbapenemase-producing Pseudomonas aeruginosa clinical isolates in Japan due to clonal expansion of ST235.
机构信息
Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
BML Inc., Kawagoe, Saitama, Japan.
出版信息
PLoS One. 2018 Nov 19;13(11):e0207134. doi: 10.1371/journal.pone.0207134. eCollection 2018.
The first outbreak in Japan of GES-5 carbapenemase-producing Pseudomonas aeruginosa occurred in a long-term care facility in 2014. To assess the spread of GES-5 producing P. aeruginosa clinical isolates in medical settings in Japan, 1,476 carbapenem-resistant P. aeruginosa isolates obtained from 2012 to 2016 were characterized. Of these 1,476 isolates, 104 (7.0%) harbored blaGES-5. Southern blotting revealed that the blaGES-5 was located on the chromosome. The isolation rates of these GES-5 producers increased significantly every year, from 2.0% (6 of 295) in 2012 to 2.8% (8 of 283) in 2013 to 5.3% (16 of 303) in 2014 to 9.7% (29 of 300) in 2015 to 15.3% (45 of 295) in 2016. Of the 104 GES-5 producers, 102 belonged to clonal complex (CC) 235, including 99 belonging to ST235 and three belonging to ST2233). Whole genome sequence analysis revealed that CC235 P. aeruginosa harboring blaGES-5 spread in a clonal manner. These results indicate that these GES-5 producing CC235 P. aeruginosa clinical isolates have spread in medical settings throughout Japan.
2014 年，日本在一家长期护理机构首次发现产 GES-5 碳青霉烯酶铜绿假单胞菌。为评估 GES-5 产铜绿假单胞菌临床分离株在日本医疗机构中的传播情况，对 2012 年至 2016 年获得的 1476 株耐碳青霉烯铜绿假单胞菌进行了特征分析。在这 1476 株分离株中，有 104 株（7.0%）携带 blaGES-5。Southern 印迹显示 blaGES-5 位于染色体上。这些 GES-5 产生者的分离率每年显著增加，从 2012 年的 2.0%（295 例中的 6 例）增加到 2013 年的 2.8%（283 例中的 8 例）、2014 年的 5.3%（303 例中的 16 例）、2015 年的 9.7%（300 例中的 29 例）和 2016 年的 15.3%（295 例中的 45 例）。在 104 株 GES-5 产生者中，有 102 株属于克隆群（CC）235，包括 99 株属于 ST235 和 3 株属于 ST2233）。全基因组序列分析显示，携带 blaGES-5 的 CC235 铜绿假单胞菌以克隆方式传播。这些结果表明，这些在医疗环境中传播的产 GES-5 CC235 铜绿假单胞菌临床分离株。
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