Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
School of Medicine, Kumamoto University, Kumamoto, Japan.
Int J Biol Macromol. 2019 Mar 1;124:282-290. doi: 10.1016/j.ijbiomac.2018.11.152. Epub 2018 Nov 16.
We examined the effects of various acidic polysaccharides isolated from marine algae on the infection and replication of human immunodeficiency virus type-1 (HIV-1), hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-cell leukemia virus type-1 (HTLV-1). It was found that sulfated fucan polysaccharides, ascophyllan, and two fucoidans derived from different sources significantly inhibited the early step of HIV-1 (R9 and JR-FL) infection, while they did not affect the late step. The alginate oligomer consisted of uronic acids and sulfated-galactan porphyran showed no significant inhibitory effects. In addition, ascophyllan and two fucoidans inhibited the early step of HBV infection in a dose-dependent manner. Furthermore, these polysaccharides inhibited the early step of HCV infection but had no inhibitory effects on HTLV-1 replication. To further examine the specificity of these polysaccharides in viral infections, we used vesicular stomatitis virus (VSV)-G-pseudotyped HIV-1 infection. Ascophyllan, the two fucoidans, and alginate oligomer also potently inhibited VSV-G-pseudotyped HIV-1 infection in HeLa cells. Taken together, these results suggest that the acidic polysaccharides used in this study are capable of inhibiting the early step of viral infections depending on the polysaccharides but not in a strict species-specific manner.
我们研究了从海洋藻类中分离得到的各种酸性多糖对人类免疫缺陷病毒 1 型（HIV-1）、乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）和人类 T 细胞白血病病毒 1 型（HTLV-1）感染和复制的影响。研究发现，硫酸岩藻聚糖、泡叶藻聚糖和两种来源不同的褐藻胶聚糖显著抑制 HIV-1（R9 和 JR-FL）感染的早期阶段，而不影响晚期阶段。由糖醛酸和硫酸化半乳聚糖 porphyran 组成的藻酸钠低聚物没有显示出显著的抑制作用。此外，泡叶藻聚糖和两种褐藻胶聚糖呈剂量依赖性地抑制 HBV 感染的早期阶段。此外，这些多糖抑制 HCV 感染的早期阶段，但对 HTLV-1 复制没有抑制作用。为了进一步研究这些多糖在病毒感染中的特异性，我们使用了水疱性口炎病毒（VSV）-G 假型 HIV-1 感染。泡叶藻聚糖、两种褐藻胶聚糖和藻酸钠低聚物也能有效地抑制 HeLa 细胞中 VSV-G 假型 HIV-1 的感染。总之，这些结果表明，本研究中使用的酸性多糖能够抑制病毒感染的早期阶段，这取决于多糖，但不是严格的种特异性方式。
