Wang Xiaoli, Xu Fanxing, Zhang Henghui, Peng Liang, Zhen Yuhong, Wang Lili, Xu Yongping, He Dongye, Li Xiaoyu
1Department of Environment and Safety Engineering, Taiyuan Institute of Technology, Taiyuan, 030008 People's Republic of China.
2School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, Dalian, 116024 People's Republic of China.
3 Biotech. 2018 Nov;8(11):473. doi: 10.1007/s13205-018-1494-6. Epub 2018 Nov 8.
The optimum extraction conditions of essential oil from var. applied by an orthogonal L(3) test were a water-to-raw material ratio of 17, a particle size of ≤ 3.8 mm and an extraction time of 2 h. In ingredient analysis, gas chromatography/mass spectrometry analysis identified the main components of essential oil as methyl eugenol (45.95%), safrole (17.48%) and 3,5-dimethoxytoluene (10.30%) orderly. In vitro, the minimum inhibitory concentration and minimum bactericidal concentration of essential oil were 0.01 and 0.02% against , 0.04 and 0.08% against , and 0.005 and 0.005% against . In vivo, administration of essential oil significantly suppressed alveolar bone resorption induced by , with bone levels remaining comparable to non-infected mice. These results of antibacterial activity of essential oil in vitro and in vivo show the inhibition of periodontal pathogens and therapy of alveolar bone resorption in mice, suggesting that its essential oil could be a potential natural therapeutic agent for treatment of periodontitis in human beings.
通过正交L(3)试验确定的从[品种名称]中提取挥发油的最佳条件为：料水比17、粒径≤3.8毫米、提取时间2小时。在成分分析中，气相色谱/质谱分析确定挥发油的主要成分依次为甲基丁香酚(45.95%)、黄樟素(17.48%)和3,5-二甲氧基甲苯(10.30%)。体外实验中，挥发油对[细菌名称1]的最低抑菌浓度和最低杀菌浓度分别为0.01%和0.02%，对[细菌名称2]为0.04%和0.08%，对[细菌名称3]为0.005%和0.005%。体内实验中，给予挥发油可显著抑制[诱导因素]诱导的牙槽骨吸收，骨水平与未感染小鼠相当。这些挥发油体外和体内抗菌活性的结果表明其对牙周病原体的抑制作用以及对小鼠牙槽骨吸收的治疗作用，提示其挥发油可能是治疗人类牙周炎的潜在天然治疗剂。
