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扫描电子显微镜分析用牛粪、满江红和土曲霉接种农作物秸秆堆肥/蚯蚓堆肥的成熟度。

Scanning electron microscopy for analysing maturity of compost/vermicompost from crop residue spiked with cattle dung, Azolla pinnata and Aspergillus terreus.

机构信息

Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.

出版信息

Environ Sci Pollut Res Int. 2019 Jan;26(2):1761-1769. doi: 10.1007/s11356-018-3673-8. Epub 2018 Nov 19.

Abstract

Rice straw and wheat straw were mixed with cattle dung (C), Azolla pinnata (A) and Aspergillus terreus (F) and subjected to aerobic composting and vermicomposting. Eight different mixtures were made as R, RC, RA, RF, RCF, RCA, RFA and RCFA and W, WC, WF, WA, WCA, WCF, WFA and WCFA. Ratio of straw and cattle dung was kept as 2:1, and weight of cattle dung was reduced proportionally when Azolla (20 g) or fungus (20 ml) was added in a mixture. Surface structural morphology of the initial and final mixtures was analysed with SEM. Initial samples showed larger particle size with a coarse surface, less compaction and arrangement of biomass as a dense meshwork of lingo-cellulosic fibres. Final samples showed smaller particle size, compaction and several pores per unit area. Electron micrographs clearly show enhancement of degradation and better texture with the addition of fungus, Azolla or cattle dung to the straw. Azolla and cattle dung when added separately, enhanced degradation and compaction, but the mixtures with fungus showed degradation with no compaction. Maximum homogeneity and smallest particle size of RCFA and WCFA indicated that addition of Azolla and fungus along with cattle dungenhanced degradation of straw and enhancement was remarkably more when the mixtures were subjected to vermicomposting. This is a first report highlighting vermicomposting of the straw mixed with Azolla and fungus along with cattle dung for obtaining a better quality product suitable for agricultural use. Parallel variation in C/N ratio and nutrient profile of the mixtures show that SEM helps in determining maturity index of composts.

摘要

稻草和麦草与牛粪(C)、满江红(A)和土曲霉(F)混合,进行好氧堆肥和蚯蚓堆肥。共制作了 8 种不同的混合物,分别为 R、RC、RA、RF、RCF、RCA、RFA 和 RCFA,以及 W、WC、WF、WA、WCA、WCF、WFA 和 WCFA。稻草和牛粪的比例保持为 2:1,当添加满江红(20 g)或真菌(20 ml)时,牛粪的重量按比例减少。用 SEM 分析初始和最终混合物的表面结构形态。初始样品的粒径较大,表面粗糙,生物质的压实度和排列程度较差,呈紧密的纤维状网络。最终样品的粒径较小,压实度较高,单位面积有几个孔。电子显微镜照片清楚地表明,在稻草中添加真菌、满江红或牛粪可增强降解作用和改善质地。单独添加满江红和牛粪可增强降解和压实度,但与真菌混合的混合物则显示出降解而无压实度。RCFA 和 WCFA 的最大均匀性和最小粒径表明,添加满江红和真菌以及牛粪可增强稻草的降解作用,而混合物进行蚯蚓堆肥时,增强效果更为显著。这是首次报道强调混合了满江红和真菌以及牛粪的稻草进行蚯蚓堆肥,以获得更适合农业用途的高质量产品。混合物中 C/N 比和养分含量的平行变化表明,SEM 有助于确定堆肥的成熟度指数。

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