Tang Chuan-Ho, Lin Ching-Yu, Tsai Yi-Lun, Lee Shu-Hui, Wang Wei-Hsien
Department of Biology, National Museum of Marine Biology and Aquarium, Pingtung, Taiwan.
Institute of Marine Biology, National Dong Hwa University, Pingtung, Taiwan.
Zoo Biol. 2018 Nov;37(6):440-451. doi: 10.1002/zoo.21452. Epub 2018 Nov 20.
Integrating multifactor blood analysis is a key step toward a precise diagnosis of the health status of marine mammals. Variations in the circulating lipid profile reflect changes in the metabolism and physiology of an individual. To demonstrate the practicability of lipid profiling for physiological assessment, the phosphorylcholine-containing lipids in the plasma of long-term managed beluga whales (Delphinapterus leucas) were profiled using a lipidomics methodology. Using a multivariate analysis, the mean corpuscular volume, cholesterol, potassium, and γ-glutamyltranspeptidase levels were well modeled with the lipid profile of the female whales. In the models, the correlated lipids provided information about blood parameter-related metabolism and physiological regulation, in particular relating to cholesterol and inflammation. In the males, the levels of cholesterol, triglycerides, blood urea nitrogen, creatinine, plasma iron, and segmented neutrophil were well modeled with the lipid profile. In addition to providing information about the related metabolism and regulation, through a cross-linked analysis of the blood parameters, the correlated lipids indicated a parallel regulation involved in the energy metabolism of the male whales. Lipidomics as a method for revealing the context of physiological change shows practical potential for the health care of managed whales.
整合多因素血液分析是精确诊断海洋哺乳动物健康状况的关键一步。循环脂质谱的变化反映了个体新陈代谢和生理机能的改变。为了证明脂质谱分析用于生理评估的实用性，采用脂质组学方法对长期圈养的白鲸（白鲸属）血浆中含磷酸胆碱的脂质进行了分析。通过多变量分析，雌性白鲸的平均红细胞体积、胆固醇、钾和γ-谷氨酰转肽酶水平与脂质谱得到了很好的模型拟合。在这些模型中，相关脂质提供了与血液参数相关的代谢和生理调节信息，特别是与胆固醇和炎症有关的信息。在雄性白鲸中，胆固醇、甘油三酯、血尿素氮、肌酐、血浆铁和分叶核中性粒细胞水平与脂质谱得到了很好的模型拟合。除了提供相关代谢和调节的信息外，通过对血液参数的交联分析，相关脂质表明雄性白鲸能量代谢中存在平行调节。脂质组学作为一种揭示生理变化背景的方法，在圈养鲸鱼的健康护理方面显示出实际潜力。
