Phurbu Dorji, Lu Hubin, Xue Xiaofeng, Tian Yanling, Pema Yangzom, Ma Chao, Xing Peng
1Tibet Plateau Institute of Biology, Lhasa 850000, PR China.
2State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, PR China.
Int J Syst Evol Microbiol. 2019 Jan;69(1):165-170. doi: 10.1099/ijsem.0.003124. Epub 2018 Nov 20.
A novel, Gram-stain-negative, non-motile and rod-shaped bacterium, designated YH5, was isolated from the YonghuCo wetland on the Tibetan Plateau. The strain was able to grow optimally with 1 % (w/v) NaCl and tolerated up to 3 % NaCl. Growth occurred at pH 6-9 (optimum pH 7) and 10-37 °C (optimum 30 °C). Vitamins were not required for growth. The major polar lipid of strain YH5 was phosphatidylethanolamine. The predominant respiratory quinone was menaquinone 6 (MK-6). The major fatty acids were iso-C15 : 0, C16 : 0 10-methyl and/or iso-C17 : 1ω9c, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH. Genome sequencing revealed a genome size of 2.74 Mbp and a G+C content of 33.3 mol%. Analysis of 16S rRNA gene sequences showed that strain YH5 belonged to the genus Flavobacterium, with the closest neighbours Flavobacterium luticocti xz20 (96.7 % similarity), Flavobacterium jejuense EC11 (96.4 %), Flavobacterium jumunjinense HME7102 (95.9 %) and Flavobacterium dongtanense LW30 (95.6 %). DNA-DNA relatedness between strain YH5 and the closest phylogenetically related strain F. luticocti xz20 was 27.0 %. Strain YH5 was clearly distinguished from the reference type strains based on phylogenetic analysis, DNA-DNA hybridization, fatty acid composition and a range of physiological and biochemical characteristics. Based on its phenotypic and chemotaxonomic characteristics, strain YH5 is classified as a representative of a novel species of the genus Flavobacterium, for which the name Flavobacterium tibetense sp. nov. is proposed. The type strain is YH5 (=CICC 24247=KCTC 62174).
从青藏高原雍湖错湿地分离出一株新型革兰氏阴性、无运动性的杆状细菌，命名为YH5。该菌株在含1%（w/v）NaCl的条件下生长最佳，可耐受高达3%的NaCl。在pH 6 - 9（最适pH 7）和10 - 37 °C（最适30 °C）条件下生长。生长不需要维生素。菌株YH5的主要极性脂是磷脂酰乙醇胺。主要呼吸醌是甲基萘醌6（MK - 6）。主要脂肪酸为异C15 : 0、C16 : 0 10 - 甲基和/或异C17 : 1ω9c、异C17 : 0 3 - OH、异C15 : 1 G和异C15 : 0 3 - OH。基因组测序显示基因组大小为2.74 Mbp，G + C含量为33.3 mol%。16S rRNA基因序列分析表明，菌株YH5属于黄杆菌属，与最相近的邻居黄杆菌luticocti xz20（相似度96.7%）、济州黄杆菌EC11（96.4%）、巨文津黄杆菌HME7102（95.9%）和东滩黄杆菌LW30（95.6%）。菌株YH5与系统发育关系最密切的菌株F. luticocti xz20之间的DNA - DNA相关性为27.0%。基于系统发育分析、DNA - DNA杂交、脂肪酸组成以及一系列生理生化特征，菌株YH5与参考模式菌株明显区分开来。基于其表型和化学分类特征，菌株YH5被归类为黄杆菌属一个新物种的代表，为此提出黄杆菌西藏种（Flavobacterium tibetense sp. nov.）的名称。模式菌株为YH5（=CICC 24247 = KCTC 62174）。
