Emerg Infect Dis. 2018 Dec;24(12):2262-2269. doi: 10.3201/eid2412.180272.
Enterohemorrhagic Escherichia coli serogroup O80, involved in hemolytic uremic syndrome associated with extraintestinal infections, has emerged in France. We obtained circularized sequences of the O80 strain RDEx444, responsible for hemolytic uremic syndrome with bacteremia, and noncircularized sequences of 35 O80 E. coli isolated from humans and animals in Europe with or without Shiga toxin genes. RDEx444 harbored a mosaic plasmid, pR444_A, combining extraintestinal virulence determinants and a multidrug resistance-encoding island. All strains belonged to clonal complex 165, which is distantly related to other major enterohemorrhagic E. coli lineages. All stx-positive strains contained eae-ξ, ehxA, and genes characteristic of pR444_A. Among stx-negative strains, 1 produced extended-spectrum β-lactamase, 1 harbored the colistin-resistance gene mcr1, and 2 possessed genes characteristic of enteropathogenic and pyelonephritis E. coli. Because O80-clonal complex 165 strains can integrate intestinal and extraintestinal virulence factors in combination with diverse drug-resistance genes, they constitute dangerous and versatile multidrug-resistant pathogens.
产志贺毒素肠出血性大肠杆菌 O80 血清群与肠外感染相关的溶血性尿毒症综合征有关，已在法国出现。我们获得了引起伴有菌血症的溶血性尿毒症综合征的 O80 菌株 RDEx444 的环形序列，以及来自欧洲人和动物的 35 株非环形 O80 大肠杆菌的序列，这些人与动物的菌株无论是否携带志贺毒素基因都有 O80 大肠杆菌。RDEx444 携带一个镶嵌质粒 pR444_A，该质粒结合了肠外毒力决定因素和一个多药耐药基因岛。所有菌株都属于克隆复合体 165，与其他主要肠出血性大肠杆菌谱系关系较远。所有 stx 阳性菌株都含有 eae-ξ、ehxA 和 pR444_A 的特征基因。在 stx 阴性菌株中，1 株产生了超广谱β-内酰胺酶，1 株携带粘菌素耐药基因 mcr1，2 株含有肠致病性和肾盂肾炎大肠杆菌的特征基因。由于 O80-克隆复合体 165 菌株可以整合肠内和肠外毒力因子，并结合多种耐药基因，因此它们构成了危险且多功能的多药耐药病原体。
