2010-2017 年瑞士人源分离的新型 O80:H2 型产志贺毒素大肠杆菌的遗传特征分析。
Genetic characterization of Shiga toxin producing Escherichia coli belonging to the emerging hybrid pathotype O80:H2 isolated from humans 2010-2017 in Switzerland.
机构信息
Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Switzerland.
Division of Clinical Microbiology, University Hospital Basel, Basel, Switzerland; Applied Microbiology Research, Department of Biomedicine, University of Basel, Switzerland.
出版信息
Int J Med Microbiol. 2018 Jul;308(5):534-538. doi: 10.1016/j.ijmm.2018.05.007. Epub 2018 Jun 1.
Shiga toxin-producing E. coli (STEC) O80:H2 is an uncommon hybrid pathotype that has recently emerged in France. We analysed 18 STEC O80:H2 isolated from humans in Switzerland during 2010-2017. All isolates carried stx2a or stx2d, the rare eae variant eae-ξ and at least seven virulence genes associated with pS88, a plasmid that is found in extraintestinal pathogenic E. coli (ExPEC). Whole genome sequencing (WGS) identified additional chromosomal extraintestinal virulence genes encoding for type 1 fimbria (fimA, fimC and fimH), aerobactin (iuc/iutA) and afimbrial adhesins (afaA/C/D/E-VIII). Core genome multi-locus sequence typing (cgMLST) detected two closely related but distinct subclusters with different stx2 and iuc/iutA genotypes. All isolates were multidrug resistant (MDR), but susceptible to third generation cephalosporins and azithromycin. STEC/ExPEC hybrid pathotypes such as STEC O80:H2 represent a therapeutical challenge in the event of extraintestinal infection.
产志贺毒素大肠杆菌（STEC）O80：H2 是一种罕见的混合血清型，最近在法国出现。我们分析了 2010 年至 2017 年期间瑞士从人类身上分离出的 18 株 STEC O80：H2。所有分离株均携带 stx2a 或 stx2d、罕见的 eae 变体 eae-ξ 和至少七种与 pS88 相关的毒力基因，pS88 是存在于肠外致病性大肠杆菌（ExPEC）中的质粒。全基因组测序（WGS）鉴定出额外的染色体肠外毒力基因，编码类型 1菌毛（fimA、fimC 和 fimH）、aerobactin（iuc/iutA）和无菌毛黏附素（afaA/C/D/E-VIII）。核心基因组多位点序列分型（cgMLST）检测到两个密切相关但不同的亚群，具有不同的 stx2 和 iuc/iutA 基因型。所有分离株均为多药耐药（MDR），但对第三代头孢菌素和阿奇霉素敏感。STEC/ExPEC 混合血清型，如 STEC O80：H2，在发生肠外感染时代表着治疗上的挑战。
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