Seelmeier S, Schmidt H, Turk V, von der Helm K
Max von Pettenkofer Institute, University of Munich, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6612-6. doi: 10.1073/pnas.85.18.6612.
The protease encoded by the human immunodeficiency virus (HIV) processes the viral gag and gag-pol protein precursor by posttranslational cleavage. In this study we have demonstrated by site-specific mutagenesis (Asp----Thr) and by pepstatin A inhibition that the recombinant HIV protease is an aspartic-type protease. Furthermore, incubation of HIV-infected H9 cells with pepstatin A inhibited part of the intracellular processing of the HIV gag protein yet had no apparent toxicity on HIV-infected cells during 48 hr of incubation.
人类免疫缺陷病毒（HIV）编码的蛋白酶通过翻译后切割处理病毒的gag和gag-pol蛋白前体。在本研究中，我们通过位点特异性诱变（天冬氨酸→苏氨酸）和胃蛋白酶抑制剂A抑制实验证明，重组HIV蛋白酶是一种天冬氨酸型蛋白酶。此外，用胃蛋白酶抑制剂A孵育HIV感染的H9细胞，可抑制HIV gag蛋白的部分细胞内加工过程，但在48小时的孵育期间对HIV感染的细胞没有明显毒性。
