Cryo-EM structure of Type III-A CRISPR effector complex.
作者信息
Huo Yangao, Li Tao, Wang Nan, Dong Qinghua, Wang Xiangxi, Jiang Tao
机构信息
National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
School of Life Science and Engineering, Foshan University, Guangdong, China.
出版信息
Cell Res. 2018 Dec;28(12):1195-1197. doi: 10.1038/s41422-018-0115-6. Epub 2018 Nov 21.
Abstract
摘要
相似文献
1
Cryo-EM structure of Type III-A CRISPR effector complex.III-A型CRISPR效应复合物的冷冻电镜结构
Cell Res. 2018 Dec;28(12):1195-1197. doi: 10.1038/s41422-018-0115-6. Epub 2018 Nov 21.
2
Structural insights into Cas13b-guided CRISPR RNA maturation and recognition.Cas13b引导的CRISPR RNA成熟与识别的结构见解
Cell Res. 2018 Dec;28(12):1198-1201. doi: 10.1038/s41422-018-0109-4. Epub 2018 Nov 13.
3
Structural basis for assembly of non-canonical small subunits into type I-C Cascade.非典型小亚基组装成 I-C 级联的结构基础。
Nat Commun. 2020 Nov 23;11(1):5931. doi: 10.1038/s41467-020-19785-8.
4
Cryo-EM structure of the type III-E CRISPR-Cas effector gRAMP in complex with TPR-CHAT.III-E型CRISPR-Cas效应器gRAMP与TPR-CHAT复合物的冷冻电镜结构
Cell Res. 2022 Dec;32(12):1128-1131. doi: 10.1038/s41422-022-00738-3. Epub 2022 Oct 24.
5
Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.通过 cryo-EM 和生物化学揭示 III-A 型 CRISPR-Csm 中 ssRNA 切割与 DNase 活性的偶联。
Cell Res. 2019 Apr;29(4):305-312. doi: 10.1038/s41422-019-0151-x. Epub 2019 Feb 27.
6
Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.冷冻电镜结构揭示CRISPR-Cas监测复合物靶向DNA的机制及抑制作用
Cell. 2017 Oct 5;171(2):414-426.e12. doi: 10.1016/j.cell.2017.09.006.
7
Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM.X 射线晶体学和 cryo-EM 确定的 III-A 型 CRISPR 效应子亚基复合物的结构组织。
Nucleic Acids Res. 2019 Apr 23;47(7):3765-3783. doi: 10.1093/nar/gkz079.
8
Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity.III-A 型 CRISPR-Cas Csm 复合物：组装、周期性 RNA 切割、DNase 活性调节和自身免疫。
Mol Cell. 2019 Jan 17;73(2):264-277.e5. doi: 10.1016/j.molcel.2018.11.007. Epub 2018 Nov 29.
9
Structure Reveals a Mechanism of CRISPR-RNA-Guided Nuclease Recruitment and Anti-CRISPR Viral Mimicry.结构揭示了 CRISPR-RNA 引导的核酸酶募集和抗 CRISPR 病毒模拟的机制。
Mol Cell. 2019 Apr 4;74(1):132-142.e5. doi: 10.1016/j.molcel.2019.02.001. Epub 2019 Mar 11.
10
Cryo-EM structure of the RNA-guided ribonuclease Cas12g.RNA 引导的核糖核酸酶 Cas12g 的冷冻电镜结构
Nat Chem Biol. 2021 Apr;17(4):387-393. doi: 10.1038/s41589-020-00721-2. Epub 2021 Jan 25.
引用本文的文献
1
Cas7 meets Cas14: a strategic partnership in the type VII CRISPR-Cas.Cas7与Cas14相遇：VII型CRISPR-Cas中的战略伙伴关系。
Protein Cell. 2025 Feb 1;16(2):79-82. doi: 10.1093/procel/pwae056.
2
Functional and Phylogenetic Diversity of Cas10 Proteins.Cas10 蛋白的功能和系统发育多样性。
CRISPR J. 2023 Apr;6(2):152-162. doi: 10.1089/crispr.2022.0085. Epub 2023 Mar 13.
3
Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector.III-E 型 CRISPR-Cas 效应蛋白的冷冻电镜结构和蛋白酶活性
Nat Microbiol. 2023 Mar;8(3):522-532. doi: 10.1038/s41564-022-01316-4. Epub 2023 Jan 26.
4
Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase.III-E 型 CRISPR 核酸酶-蛋白酶 Caspase 非自身 RNA 激活蛋白酶活性的结构基础。
Nat Commun. 2022 Dec 7;13(1):7549. doi: 10.1038/s41467-022-35275-5.
5
Structures of an active type III-A CRISPR effector complex.一种活性 III-A 型 CRISPR 效应复合物的结构。
Structure. 2022 Aug 4;30(8):1109-1128.e6. doi: 10.1016/j.str.2022.05.013. Epub 2022 Jun 16.
6
Structural and biochemical characterization of in vivo assembled Lactococcus lactis CRISPR-Csm complex.体内组装的乳球菌 CRISPR-Csm 复合物的结构和生化特性。
Commun Biol. 2022 Mar 29;5(1):279. doi: 10.1038/s42003-022-03187-1.
7
Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.基于结构的抗 CRISPR 蛋白的功能机制和生物技术应用。
Nat Rev Mol Cell Biol. 2021 Aug;22(8):563-579. doi: 10.1038/s41580-021-00371-9. Epub 2021 Jun 4.
8
Characterization of a novel type III CRISPR-Cas effector provides new insights into the allosteric activation and suppression of the Cas10 DNase.一种新型III型CRISPR-Cas效应器的特性研究为Cas10 DNA酶的变构激活和抑制提供了新见解。
Cell Discov. 2020 May 12;6:29. doi: 10.1038/s41421-020-0160-4. eCollection 2020.
9
Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials: Review and Prospects.基于内源性CRISPR-Cas系统的基因组编辑与抗菌剂：综述与展望
Front Microbiol. 2019 Oct 25;10:2471. doi: 10.3389/fmicb.2019.02471. eCollection 2019.
10
CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA Cleavage with ApA>p Formation Terminating RNase Activity.CRISPR-Cas III-A Csm6的CARF结构域是一种环状核酸酶，可引发逐步的cA切割，形成ApA>p从而终止核糖核酸酶活性。
Mol Cell. 2019 Sep 5;75(5):944-956.e6. doi: 10.1016/j.molcel.2019.06.014. Epub 2019 Jul 17.
本文引用的文献
1
Type III CRISPR-Cas systems produce cyclic oligoadenylate second messengers.III 型 CRISPR-Cas 系统产生环状寡聚腺苷酸第二信使。
Nature. 2017 Aug 31;548(7669):543-548. doi: 10.1038/nature23467. Epub 2017 Jul 19.
2
A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems.III 型 CRISPR-Cas 系统中的环状寡核苷酸信号通路。
Science. 2017 Aug 11;357(6351):605-609. doi: 10.1126/science.aao0100. Epub 2017 Jun 29.
3
RNA activation-independent DNA targeting of the Type III CRISPR-Cas system by a Csm complex.Csm复合物对III型CRISPR-Cas系统进行不依赖RNA激活的DNA靶向
EMBO Rep. 2017 May;18(5):826-840. doi: 10.15252/embr.201643700. Epub 2017 Mar 31.
4
Type III CRISPR-Cas Immunity: Major Differences Brushed Aside.III 型 CRISPR-Cas 免疫：主要差异被忽略。
Trends Microbiol. 2017 Jan;25(1):49-61. doi: 10.1016/j.tim.2016.09.012. Epub 2016 Oct 20.
5
Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition.III-A 型 CRISPR-Cas 免疫的时空调控：将 DNA 降解与靶标 RNA 识别相偶联。
Mol Cell. 2016 Apr 21;62(2):295-306. doi: 10.1016/j.molcel.2016.03.024.
6
An updated evolutionary classification of CRISPR-Cas systems.CRISPR-Cas系统的最新进化分类
Nat Rev Microbiol. 2015 Nov;13(11):722-36. doi: 10.1038/nrmicro3569. Epub 2015 Sep 28.
7
Crystal structure of the CRISPR-Cas RNA silencing Cmr complex bound to a target analog.CRISPR-Cas RNA 沉默 Cmr 复合物与靶类似物结合的晶体结构。
Mol Cell. 2015 May 7;58(3):418-30. doi: 10.1016/j.molcel.2015.03.018. Epub 2015 Apr 23.
8
Structural biology. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.结构生物学。CRISPR-Cmr复合物的结构揭示了RNA靶标定位模式。
Science. 2015 May 1;348(6234):581-5. doi: 10.1126/science.aaa4535. Epub 2015 Apr 2.
9
Crystal structure of the Csm1 subunit of the Csm complex and its single-stranded DNA-specific nuclease activity.Csm 复合物的 Csm1 亚基的晶体结构及其单链 DNA 特异性核酸酶活性。
Structure. 2015 Apr 7;23(4):782-90. doi: 10.1016/j.str.2015.01.021. Epub 2015 Mar 12.
10
RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus.嗜热栖热菌III-A型CRISPR-Cas Csm复合物对RNA的靶向作用
Mol Cell. 2014 Nov 20;56(4):518-30. doi: 10.1016/j.molcel.2014.10.005. Epub 2014 Nov 6.
Zotero 插件