Watcharawipas Akaraphol, Watanabe Daisuke, Takagi Hiroshi
Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara, Japan.
Front Microbiol. 2018 Oct 16;9:2495. doi: 10.3389/fmicb.2018.02495. eCollection 2018.
Recent studies have revealed the feasibility of sodium acetate as a potentially novel inhibitor/stressor relevant to the fermentation from neutralized lignocellulosic hydrolysates. This mini-review focuses on the toxicity of sodium acetate, which is composed of both sodium and acetate ions, and on the involved cellular responses that it elicits, particularly via the high-osmolarity glycerol (HOG) pathway, the Rim101 pathway, the P-type ATPase sodium pumps Ena1/2/5, and the ubiquitin ligase Rsp5 with its adaptors. Increased understanding of cellular responses to sodium acetate would improve our understanding of how cells respond not only to different stimuli but also to composite stresses induced by multiple components (e.g., sodium and acetate) simultaneously. Moreover, unraveling the characteristics of specific stresses under industrially related conditions and the cellular responses evoked by these stresses would be a key factor in the industrial yeast strain engineering toward the increased productivity of not only bioethanol but also advanced biofuels and valuable chemicals that will be in demand in the coming era of bio-based industry.
最近的研究表明，醋酸钠作为一种潜在的新型抑制剂/应激源，对于从中性化木质纤维素水解产物进行发酵具有可行性。本综述聚焦于由钠离子和醋酸根离子组成的醋酸钠的毒性，以及它所引发的细胞反应，特别是通过高渗甘油（HOG）途径、Rim101途径、P型ATP酶钠泵Ena1/2/5以及泛素连接酶Rsp5及其衔接蛋白所引发的反应。对细胞对醋酸钠反应的深入理解，将有助于我们不仅了解细胞如何对不同刺激做出反应，还能了解其如何对由多种成分（如钠离子和醋酸根离子）同时诱导的复合应激做出反应。此外，揭示工业相关条件下特定应激的特征以及这些应激所引发的细胞反应，将是工业酵母菌株工程中提高生物乙醇、先进生物燃料以及未来生物基产业所需的有价值化学品生产率的关键因素。
