Lin Xiangna, Xia Yongjun, Wang Guangqiang, Yang Yijin, Xiong Zhiqiang, Lv Fang, Zhou Wei, Ai Lianzhong
Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China.
Yangzhou Yangda Kangyuan Dairy Ltd., Yangzhou, China.
Front Microbiol. 2018 Nov 6;9:2684. doi: 10.3389/fmicb.2018.02684. eCollection 2018.
In order to screening new Lactic acid bacteria (LAB) strains to alleviating liver injury induced by oxidized oil, we isolated and screened LAB from Chinese fermented foods. AR113, AR243, and AR501 showed higher scavenging activity of α, α-Diphenyl-β-Picrylhydrazyl (DPPH) free radical and hydrogen radical, stronger inhibition of lipid peroxidation, and better protective effect on yeast cells . , oral administration of AR501 improved the antioxidant status of injury mice induced by oxidized oil including decreasing lipid peroxidation, recovering activities of antioxidant enzymes. Meanwhile, the gene expression of Nuclear factor erythroid 2-related factor 2 (Nrf2) of AR501 group was markedly elevated, and several antioxidant genes such as glutathione S-transferase (GSTO1), heme oxygenase-1 (HO-1), Glutamate cysteine ligase (GCL), and NAD(P)H:quinone oxidoreductase-l (NQO1) were subsequently up regulated in mice liver. Therefore, AR501 could be considered as potential candidates for production of functional foods that can alleviate the oxidative damage induced by oxidized oil.
为了筛选新的乳酸菌（LAB）菌株以减轻氧化油诱导的肝损伤，我们从中国发酵食品中分离并筛选了乳酸菌。AR113、AR243和AR501表现出较高的α，α-二苯基-β-苦基肼（DPPH）自由基和氢自由基清除活性，对脂质过氧化的抑制作用更强，对酵母细胞的保护作用更好。此外，口服AR501可改善氧化油诱导的损伤小鼠的抗氧化状态，包括降低脂质过氧化，恢复抗氧化酶活性。同时，AR501组的核因子红细胞2相关因子2（Nrf2）基因表达明显升高，随后小鼠肝脏中的几种抗氧化基因如谷胱甘肽S-转移酶（GSTO1）、血红素加氧酶-1（HO-1）、谷氨酸半胱氨酸连接酶（GCL）和NAD（P）H：醌氧化还原酶-1（NQO1）也上调。因此，AR501可被视为生产能减轻氧化油诱导的氧化损伤的功能性食品的潜在候选菌株。
