He Yuanyuan, Li Feiran, Zhang Yu, Zhu Xiaohui, Lin Zhengrong, Li Linxiao, Nawaz Shah, Kulyar Muhammad Fakhar-E-Alam, Iqbal Mudassar, Li Jiakui
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
College of Animals Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Linzhi, Tibet, 860000, People's Republic of China.
Probiotics Antimicrob Proteins. 2024 Jul 24. doi: 10.1007/s12602-024-10324-1.
Chemotherapy-induced intestinal mucositis based on 5-fluorouracil (5-FU) slows down the progress of cancer treatment and causes significant suffering to patients. Pediococcus pentosaceus (P. pentosaceus), as a type of LAB, has a range of probiotic properties, including antioxidant, immune benefits, and cholesterol-lowering effects, which are attracting increasing attention. However, studies on the protective effect of P. pentosaceus against chemotherapeutic-induced intestinal mucositis caused by 5-FU remain unclear. Therefore, this study aimed to investigate the potential relieving effects of P. pentosaceus PP34 on 5-FU-induced intestinal mucositis and its mechanism. In the present study, a P. pentosaceus PP34 solution (2 × 10 CFU/mL) was administered daily by gavage followed by intraperitoneal injection of 5-FU to model intestinal mucositis. The body weight, serum biochemical indices, jejunal pathological organization, and expression levels of inflammatory cytokines in the jejunum were examined. The results indicated that the mice induced with 5-FU developed typical intestinal mucositis symptoms and histopathological changes with intense inflammatory and oxidative responses. Moreover, the gut microbiota was disturbed, while PP34 effectively decreased the oxidative reactions and the expression levels of inflammatory mediators and regulated the gut microbiota in 5-FU-exposed mice. Taken together, the study indicated that P. pentosaceus PP34 ameliorates 5-Fluorouracil-induced intestinal mucositis via inhibiting oxidative stress and restoring the gut microbiota.
基于5-氟尿嘧啶（5-FU）的化疗诱导的肠道黏膜炎会减缓癌症治疗进程，并给患者带来巨大痛苦。戊糖片球菌作为一种乳酸菌，具有一系列益生菌特性，包括抗氧化、免疫调节和降胆固醇作用，正受到越来越多的关注。然而，关于戊糖片球菌对5-FU引起的化疗诱导肠道黏膜炎的保护作用的研究仍不明确。因此，本研究旨在探讨戊糖片球菌PP34对5-FU诱导的肠道黏膜炎的潜在缓解作用及其机制。在本研究中，每天通过灌胃给予戊糖片球菌PP34溶液（2×10⁹ CFU/mL），随后腹腔注射5-FU以建立肠道黏膜炎模型。检测小鼠体重、血清生化指标、空肠病理组织以及空肠中炎性细胞因子的表达水平。结果表明，5-FU诱导的小鼠出现了典型的肠道黏膜炎症状和组织病理学变化，伴有强烈的炎症和氧化反应。此外，肠道微生物群受到干扰，而PP34有效地降低了氧化反应和炎性介质的表达水平，并调节了5-FU处理小鼠的肠道微生物群。综上所述，该研究表明戊糖片球菌PP34通过抑制氧化应激和恢复肠道微生物群来改善5-氟尿嘧啶诱导的肠道黏膜炎。
